Why is EGTA used in a CaM agarose pull down assay? Why will there be no...
Why is EGTA used in a CaM agarose pull down assay?
Why will there be no binding of gene with CaM in EGTA solution?
Homework Answers
1.EGTA is chelating agent which binds to calcium ions and chelate it with high affinity so it used to analyze whether the target protein is calcium dependent or independent
2. Only specific target protein binds to calmodin (CaM) in presence of calcium in EGTA solution. Gene will not bind.
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Why is EGTA used in a CaM agarose pull down assay?
Why will there be no...
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