What is post-translational processing, and why is it important for protein function?
Post translational processing is a biochemical modification that occurs to one or more amino acids on a protein after the protein has been translated by a ribosome.
It plays a fundamental role in regulating the folding of proteins, their targeting to specific subcellular compartment , their interaction with ligands or other proteins.
What is post-translational processing, and why is it important for protein function?
Describe the following: 1) how the function of pRb protein is regulated by post-translational modifications during the normal mitotic cell cycle and 2) how the modifications control the normal cell cycle progression through the R-point and initiation of the S-phase of the cell cycle.
Which of the following is an example of post-translational control? A protein is phosphorylated on a particular amino acid. An activator protein binds to an enhancer and interacts with another protein bound near the promoter. O A ribosome is prevented from binding to the mRNA. ODNA becomes tightly associated with histones.
is to post-translational ER protein import. O PDI (multiple choice) Mitochondrial hsp 70 is to matrix protein import what Ribosome Osec61 Osec63 O BiP 9. O co
Explain an example of post-translational modification of enzyme activity using examples from plants? Why is post-translational modification of proteins useful in cells? (4) Explain how extremes of cold or heat affect the balance between the light and dark (light-independent) parts of photosynthesis (use a diagram) (5)
Why is important (for determining the function of a membrane protein) to know if it spans the bilayer or appears only on one face of the membrane?
Some proteins can function as enzymes. How does a protein become phosphorylated? What effect can this post-translational modification (PTM) have on a protein?
You prepared a translational fusion of protein Z and the yellow fluorescent protein YFP (ZYFP) then expressed it in an animal cell line. You hypothesize that protein Z is a component of two different complexes in a cell. To test this idea, you intend to image Z-YFP using a fluorescence microscope equipped with a 60x objective (NA=0.9), and a 540 nm filter to detect YFP emission. Can you distinguish these complexes if they are approximately 330 nm apart? Explain why...
Explain the difference between translational, post-translational, transcriptional, and post-transcriptional gene regulation. Give brief examples of each.
What is the difference between a transcriptional and a translational start site? A) there is no difference-these terms are interchangeable B) The transcriptional start site is used to initiate the production of the polypeptide while translation start site is used to initiate the production of the initial RNA transcript C) The translational start site is important for determining the half-life of the RNA D) The translational start site is used to initiate the production of the polypeptide while transcriptional start...
Where does the following regulation happen? •transcriptional regulation •post-transcriptional regulation •post-translational regulation •miRNA/siRNA (mRNA degradation) •Ubiquitination/phosphorylation (protein degradation)