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biochem lab- if im trying to dialyze unpasteurized milk to seperate the AP out, what buffer...

biochem lab- if im trying to dialyze unpasteurized milk to seperate the AP out, what buffer would I use?
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Answer #1

Answer-

Tris HCl (Tris Hydrochloride) buffer can be used to seperate alkaline phosphatase from unpasteurized milk.

Preparation-

-> Dissolve 121.14 gram of Tris in 800 ml of dilute H2O, and then adjust its pH by adding appropriate amount of concentrated HCl, then add distilled water to make up 1 litre of solution, Autoclave the solution and keep it in room temperature.

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