Consider the synthesis of mRNA in bacteria. What enzyme is needed for its synthesis? What confers...
Consider the synthesis of mRNA in bacteria.
- What enzyme is needed for its synthesis?
- What confers this enzyme specificity for particular gene families?
- Where on the DNA does this enzyme begin its activity?
- What is the enzyme’s direction of polymerization?
- What mechanisms terminate this enzyme’s activity?
Homework Answers
DNA dependent RNA polymerase is used for the synthesis of messenger RNA in bacteria.
This enzyme belongs to a gene family which encodes for polymerase enzymes. All of these enzymes carry out polymerization of monomers.
It begins its activity on specific DNA sequence called as promoter.
The direction of polymerization is 5' to 3'.
The mechanism of termination of the enzyme activity are rho dependent and rho independent. In the former, rho protein binding to the enzyme so that it terminates its activity and in the latter, RNA being formed itself forms a hair pin structure so that RNA polymerase cannot move ahead.
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Consider the synthesis of mRNA in bacteria.
What enzyme is needed for its synthesis?
What confers...
#59) During protein synthesis, what happens to the ribosome? A) attach to the mRNA molecule and...
#59) During protein synthesis, what happens to the ribosome? A) attach to the mRNA molecule and travel along its length B) attach to the DNA molecule and travel along its length to produce an mRNA molecule C) Translate mRNA into tRNA D) Transcribe mRNA to tRNA E) Translate mRNA into DNA #60) if you digested the human genome (3 billion bp) with a 7-cutter restriction enzyme, approximately how many fragments would you get? A) 511461 B)183105 C)16384 D)88 E) None...
pls fo all 20) A) an enzyme that synthesizes RNA as part of the transcription process...
pls fo all
20) A) an enzyme that synthesizes RNA as part of the transcription process B) an enzyme that uses RNA as a substrate C) an enzyme that catalyzes the association between the large and small ribosomal subunits D) an enzyme that synthesizes RNA primers during DNA replication E) an RNA with enzymatic activity 20) What is a ribozyme? 21) 21) Alternative RN A splicing A) increases the rate of transcription. B) can allow the production of similar proteins...
2. When transcribing an mRNA strand, RNA polymerase uses the strand of DNA to match complementary...
2. When transcribing an mRNA strand, RNA polymerase uses the strand of DNA to match complementary bases with. RNA polymerase always reads this strand in the direction and always builds mRNA in the direction. (1.5 pts) 3. (0.5 pt) What is the significance of the +1 site in regards to transcription of mRNA? t) When translating an mRNA sequence, where does the ribosome always begin? 5. (0.5 pt) When translating an mRNA sequence, what signals the ribosome to end translation?...
Background Information How can we predict where a coding gene will be in bacteria? And can...
Background Information How can we predict where a coding gene will be in bacteria? And can we then predict what protein will be produced? Take the DNA sequence below, for example. tcaggctttaattcatccgtgatctttgacgacggtaaatacgatgcagatataatacgatgaccgatgccaatcgaccgatcaaggaggcaccgaatggcgatgatggcgatgattgcgattaacgaagtggaacgcattatggcgggcattaacgaagatacccatgcgaccggcgaaaacgaaaccatttgcagctgcgcgaactttgaagaactgacccatgcgaccggccgcgaagcgacctaaaagtcgtaattacgtatcaagtcatgggccgcgggcgcccggcccactgactagactagggccgggcgcccgcggcccaccatataaataaaaaaaaaaaaaacgaggctatagctcatcaatgacct If you were a bacterial RNA polymerase, what sequence(s) should there be in this DNA for you to bind and begin transcribing? And if you found such sequence(s), where would you begin transcription? As a human being looking at this fragment of DNA, what type of consensus sequence(s)...
26. Synthesis of a repressible enzyme is stopped by: a. the allosteric transition. b. the substrate...
26. Synthesis of a repressible enzyme is stopped by: a. the allosteric transition. b. the substrate binding to the repressor c. the corepressor (product) binding to the operator d. the corepressor-repressor (product-repressor) complex binding to the operator. e. the end-product binding to the promoter 27. Glucose effect or catabolite repression is an inhibition of the metabolism of alternative carbon sources by glucose Truel False 28. microRNA regulate gene expression on post-transcriptional level True False 29. A low rate of spontaneous...
not sure what the correct answers are To be export-ready, an mRNA molecule must: have its...
not sure what the correct answers are
To be export-ready, an mRNA molecule must: have its 5' cap removed be bound by EJCS have its poly-A tail removed have at least half of its introns removed od 80e covou The extension temperature used during PCR is determined by: the length of the PCR product you want to generate the DNA polymerase that is being used the annealing temperature of your primers x the source of your template DNA (eg, genomic...
Match the enzyme activity in DNA synthesis with its function. DNA pol III (_) fragments Helicase...
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7. In bacteria the elongation stage of protein synthesis does not involve: A) aminoacyl-tRNAS B) EF-Tu...
7. In bacteria the elongation stage of protein synthesis does not involve: A) aminoacyl-tRNAS B) EF-Tu C) GTP D) IF-2 E) peptidyl transferase Which of the following best describes the cholesterol molecule? A) Amphipathic B) Nonpolar, charged C) Nonpolar, uncharged D) Polar, charged E) Polar, uncharged The enzyme that attaches an amino acid to a tRNA (aminoacyl-tRNA synthetase): A) always recognizes only one specific tRNA. B) attaches a specific amino acid to any available tRNA species. C) attaches the amino...
25. What binds to a stop codon on a mRNA during translation? a. transcription factor c....
25. What binds to a stop codon on a mRNA during translation? a. transcription factor c. termination factor b. tRNA d. transcription initiator 26. What is typically attached to the acceptor end of a tRNA? a. a protein b. an amino acid C a ribosome d. a nucleosome 27. During mRNA processing, what is put on the 3' end of a primary mRNA transcript? a. a poly-A tail b. a cap d. an intron c. an exon 28. Which of...
Can someone please explain this, I know its 5 -->3 then mRNA is 3' -->5' but...
Can someone please explain this, I know its 5 -->3 then mRNA
is 3' -->5' but how and where does the tRNA come into play? I
thought the aminos/polypeptides where next?
A particular triplet of bases in the template strand of a coding sequence of DNA is 3-AAA-5. What is the anticodon on the tRNA that binds the corresponding mRNA codon? 5-UUU-3 O 5-TTT-3 5-UUA-3 5-AAA-3
pls fo all
20) A) an enzyme that synthesizes RNA as part of the transcription process B) an enzyme that uses RNA as a substrate C) an enzyme that catalyzes the association between the large and small ribosomal subunits D) an enzyme that synthesizes RNA primers during DNA replication E) an RNA with enzymatic activity 20) What is a ribozyme? 21) 21) Alternative RN A splicing A) increases the rate of transcription. B) can allow the production of similar proteins...
2. When transcribing an mRNA strand, RNA polymerase uses the strand of DNA to match complementary bases with. RNA polymerase always reads this strand in the direction and always builds mRNA in the direction. (1.5 pts) 3. (0.5 pt) What is the significance of the +1 site in regards to transcription of mRNA? t) When translating an mRNA sequence, where does the ribosome always begin? 5. (0.5 pt) When translating an mRNA sequence, what signals the ribosome to end translation?...
26. Synthesis of a repressible enzyme is stopped by: a. the allosteric transition. b. the substrate binding to the repressor c. the corepressor (product) binding to the operator d. the corepressor-repressor (product-repressor) complex binding to the operator. e. the end-product binding to the promoter 27. Glucose effect or catabolite repression is an inhibition of the metabolism of alternative carbon sources by glucose Truel False 28. microRNA regulate gene expression on post-transcriptional level True False 29. A low rate of spontaneous...
not sure what the correct answers are
To be export-ready, an mRNA molecule must: have its 5' cap removed be bound by EJCS have its poly-A tail removed have at least half of its introns removed od 80e covou The extension temperature used during PCR is determined by: the length of the PCR product you want to generate the DNA polymerase that is being used the annealing temperature of your primers x the source of your template DNA (eg, genomic...
Match the enzyme activity in DNA synthesis with its function. DNA pol III (_) fragments Helicase (_) Primase (_) using template strand DNA pol I (_) double helix DNA ligase (_) adds nucleotides Topoisomerase (_) primer A. anneals DNA B. relieves overwinding C. adds nucleotides D. unwinds parental E. removes RNA primer, F. adds a short RNA 10 Proofreading and repair of the DNA double helix does NOT involves a. detecting a mis-match in base pairs. b. removing the mis-match...
7. In bacteria the elongation stage of protein synthesis does not involve: A) aminoacyl-tRNAS B) EF-Tu C) GTP D) IF-2 E) peptidyl transferase Which of the following best describes the cholesterol molecule? A) Amphipathic B) Nonpolar, charged C) Nonpolar, uncharged D) Polar, charged E) Polar, uncharged The enzyme that attaches an amino acid to a tRNA (aminoacyl-tRNA synthetase): A) always recognizes only one specific tRNA. B) attaches a specific amino acid to any available tRNA species. C) attaches the amino...
25. What binds to a stop codon on a mRNA during translation? a. transcription factor c. termination factor b. tRNA d. transcription initiator 26. What is typically attached to the acceptor end of a tRNA? a. a protein b. an amino acid C a ribosome d. a nucleosome 27. During mRNA processing, what is put on the 3' end of a primary mRNA transcript? a. a poly-A tail b. a cap d. an intron c. an exon 28. Which of...
Can someone please explain this, I know its 5 -->3 then mRNA
is 3' -->5' but how and where does the tRNA come into play? I
thought the aminos/polypeptides where next?
A particular triplet of bases in the template strand of a coding sequence of DNA is 3-AAA-5. What is the anticodon on the tRNA that binds the corresponding mRNA codon? 5-UUU-3 O 5-TTT-3 5-UUA-3 5-AAA-3
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