Question

1-The genetic disease called alpha1 antitrypsin deficiency is caused by a mutation of the SERPINA1 gene. The version of the gene called M produces normal proteins. The S version and the Z version produce mutant forms of the protein that can lead to lung and liver damage.

            a. If someone has two copies of the M version (MM), this is their:

                                   

            Circle or Highlight ONE:                          genotype       phenotype?

2-In our lab, why did everyone have two copies of the PTC gene instead of one? Circle or Highlight ONE

                        a. PCR makes a lot of copies of DNA

                        b. because there are two phenotypes: tasters and nontasters

                        c. one copy comes from each parent

                        d. the restriction enzyme cuts the DNA into two pieces

3-What would be the effect on PCR if there are no primers in the reagent mixture?

Circle or Highlight ONE

                                                a. no DNA would be copied

                                                b. all of the DNA would be copied

                                                c. random pieces of the DNA would be copied

                                                d. none of the DNA could be denatured

                                                e. none of the DNA could be renatured

4-Which one of the following best describes a primer? Circle or Highlight ONE

            a. Single stranded DNA

            b. Double stranded DNA

            c. small circular piece of DNA that may or may not have genes on it

            d. stain that binds to DNA and makes it glow under UV light

5-At the beginning of each cycle the temperature of the PCR is raised in order to

Circle or Highlight ONE

                                    a. denature the double DNA strands

                                    b. make a longer polymer of the DNA

                                    c. attach the primer

                                    d. re-nature the DNA strands

                                    e. elongate the primer

Answer 1

1a. Genotype- MM

Phenotype- normal appearence

2c.   one copy comes from each parent

One allele for every gene in an organism is inherited from each of that organism's parents.

3 a. no DNA would be copied

The purpose of PCR primers is to provide a “free” 3'-OH group to which the DNA polymerase can add dNTPs.

4a.  Single-stranded DNA

PCR primers are short fragments of single-stranded DNA (15-30 nucleotides in length) that are complementary to DNA sequences that flank the target region of interest.

5a.    a. denature the double DNA strands

At this high temperature, the template DNA is melted or denatured.