Question

1. Which of the DNA repair mechanism can be template independent and require polymerase activity?
a) base excision repair
b) mismatch repair
c) light-dependent repair
d) error-prone repair
e) nucleotide excision repair

What does template independent mean in this case, because i am confused!

2. gene conversion of neurospora requires recombination and which of the following events?
a) reversion
b) suppression
c) photoreactivation
d) heteroduplex formation
e) inversion

3. George W. Beadle and Edward L. Tatum refined the concept of the gene to state which of the following original ideas?
a) one gene - one enzyme
b) one gene - one metabolic block
c) one gene - one transcript
d) one gene - one polypeptide
e) one gene - one allele

4. Beadle and Tatum's concept of the gene has been further refined to state which of the following most correct and current idea?
a) one gene - one enzyme
b) one gene - one polysaccharide
c) one gene - one transcript
d) one gene - one metabolic block
e) one gene - one allele

5. Which of the following is a true statement regarding the operational definition of a gene?
a) most genes encode one polypeptide and can be operationally defined by the complementation test
b) most genes encode two polypeptides and can be operationally defined by the anti-parallel test
c) most genes encode for less than one polypeptide and can be operationally defined by the complementation test
d) most genes encode for one polypeptide and can be operationally defined by the Biuret test
e) most genes encode for one polypeptide and can be operationally defined by the Fluctuation test

Please explain each answer, thankyou in advance!

Answer 1

1. d) error prone repair [ if there is complete absence of templating strand then it is called template independent.it is occur in single stranded DNA and synthesis of DNA strand occur with the help of polymerase activity ]
2. d) heteroduplex formation [ heteoduplex formation means in double stranded DNA,when two homologous chromosome consisting of single stranded gene in each chromosome then it may or may not be complementary ]
3. a) one gene- one enzyme
4. c) one gene- one transcript
5. a) most genes encode one polypeptide and can be operationally defined by the complementation test.