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The partial sequences recognized by two restriction enzymes “A” and “B” are indicated below. Complete the...

The partial sequences recognized by two restriction enzymes “A” and “B” are indicated below. Complete the sequences such that palindromes are generated for each of the sites. On your palindromes indicate which phosphodiester linkage would have to be cleaved such that 5’ protruding ends of enzyme “A” are compatible with protruding ends of enzyme “B”. Ex. CT/GCAG

Enzyme “A”: 5’ATAG― ― ― ― 3’

Enzyme “B”: 5’GCG ― ― ― 3’

Consider your answer to the previous question. A DNA fragment generated with enzyme “A” was ligated to a DNA fragment generated with enzyme “B” as illustrated below. What would be the newly sequence at the junction of the ligated fragments? Which enzymes, A, B, A & B, or neither A or B would cut the ligated DNA?

----------------------------------A/B----------------------------------

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