How do I calculate velocity using the beer's law if given the substrate is 2 mM,...
How do I calculate velocity using the beer's law if given the substrate is 2 mM, the Δabs/min is 0.68, and the reaction was monitored for a 1 min @400 nm? Can you please write this step by step for I can study from this and apply it to problems in the future. Thank you!!!
Also, pathlength is 1 cm, e is 17800/(M*cm) at 400nm.
Homework Answers
The Beer's Law says that , 
, that is ,
absorbance is proportional to the concentration .
Now , from complete Lambert-Beers Law of Spectrophotometry , we see
b= pathlength=1 cm
=17800
M-1cm-1
By differentiating with time in both sides , we get ,
V=velocity=
Now , we have to just put the values .
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How do I calculate velocity using the beer's law if given the
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