For an experiment,DCPIP is provided at a concentration of 0.15mg/ml. If you need a 20 uq...
For an experiment,DCPIP is provided at a concentration of 0.15mg/ml. If you need a 20 uq of DCPIP, what volume would you aliquot out with a pipettor?
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For an experiment,DCPIP is provided at a concentration of
0.15mg/ml. If you need a 20 uq...
Ascorbic Acid Concentration (g/L) Volume of DCPIP in Full Syringe Used (mL) Volume of DCPIP in...
Ascorbic Acid Concentration (g/L)
Volume of DCPIP in Full Syringe Used (mL)
Volume of DCPIP in Final Syringe (mL)
Total Volume of DCPIP Used (mL)
0.0
5 mL
4.9 mL
0.1 mL
0.50
5 mL
2 mL
3 mL
1.00
5 mL
1.2 mL
3.8 mL
1.50
5 mL
3.6 mL
6.4 mL
2.00
5 mL
2.8 mL
7.2 mL
Unknown Trial 1
5 mL
2.0 mL
3.0 mL
Unknown Trial 2
5 mL
2.2 mL
2.8 mL
Unknown Trial...
For your experiment, you prepared 2ml of cells with a concentration of 2x106/ml (Tube F). You...
For your experiment, you prepared 2ml of cells with a concentration of 2x106/ml (Tube F). You would like to use this cell's solution to prepare a 7ml flask (Tube G) of cells with a concentration of 5x105cells/ml. What volume in microliters of cell solution will you take from tube F into tube G?
Lab 7 7. In this experiment, DCPIP was used to indirectly measure photosynthetic rate. If you...
Lab 7 7. In this experiment, DCPIP was used to indirectly measure photosynthetic rate. If you were asked to design an experiment so that photosynthesis could be measured directly, what factors might you measure? 8. Compare your results for the absorption spectrum and the action spectrum. Explain any discrepancies between the wavelengths of light that are absorbed maximally and those wavelengths that cause the greatest photosynthetic activity. (Note that the wavelengths of light transmitted by each coloured filter can be...
You need 100 mL of a 0.00057 M solution for an experiment. You are given 10...
You need 100 mL of a 0.00057 M solution for an experiment. You are given 10 mL of a 5.7 M stock solution. What is the best method to get the final volume and concentration you for trial? -It is not possible to reach that concentration with the info presented. -Use a 1.0 mL volumetric pipette and then dilute with water to 100 mL, then repeat serially until the desired concentration is reached. -Use a graduated cylinder to measure 0.1...
You are given a protein solution with a concentration of 0.15 mg/ml. We need 0.1 µg...
You are given a protein solution with a concentration of 0.15 mg/ml. We need 0.1 µg for an experiment. What volume of the protein solution do we need?
Suppose you need to standardize a sodium thiosulfate solution for a titration experiment. To do so,...
Suppose you need to standardize a sodium thiosulfate solution for a titration experiment. To do so, you will react it with a solution of iodine. You add a 1.00 mL aliquot of 0.0200 M KIO3 solution to a flask, followed by 3 mL of distilled water, 0.2 g of solid KI, and 1 mL H2SO4. You then titrate the solution with sodium thiosulfate solution in order to determine the exact concentration of Na2S2O3. The end point of the titration is...
i need help with number 8 and 9 please! 297 Experiment 24 10. Common ion effect a. Concentration of KHT final solution (50 mL) b. Volume of solution c. Mass of KCI d. Concentration of KCI 5.0x10...
i need help with number 8 and 9 please!
297 Experiment 24 10. Common ion effect a. Concentration of KHT final solution (50 mL) b. Volume of solution c. Mass of KCI d. Concentration of KCI 5.0x10 mL 271 381 f. Total [H [KHned g. Saturation temperature (C) h. Saturation temperature (K) 516 332.7S 0428 j. Why did the temperature have to be increased to dissolve the KHT when the KCI was added? B. K, by titration. 10.1:C 1. Temperature...
1. You have a bacterial cell culture with a concentration of 1x109 cells/ml. You need to...
1. You have a bacterial cell culture with a concentration of 1x109 cells/ml. You need to dilute the cells to a concentration of 1x103. What is the correct ratio for this dilution? 2. You add 0.1 ml of a yeast culture to a test tube that contains 9.9 ml of buffer solution. What is the dilution factor of this mixture? 3. In a 1000 µl total dilution volume, the volume of cell culture used is 800 µl. What is the...
Part I: You are instructed to produce solutions of H2S04 and NaOH for your lab experiment. The concentration you should produce is shown in the table below. Complete the table and copy it into your l...
Part I: You are instructed to produce solutions of H2S04 and NaOH for your lab experiment. The concentration you should produce is shown in the table below. Complete the table and copy it into your lab notebook for use during your lab. You will be provided with a 3.0 M stock solution of H2SO4 and solid NaOH. You will need to make 100 mL of each solution. H2SO4 Compound NaOH 0.9 Solution Concentration (M) 1.12 Moles in 100 mL 112...
Prepare the buffer. In this lab we store our buffer at a concentration 20 times the...
Prepare the buffer. In this lab we store our buffer at a concentration 20 times the concentration used in the experiment. Thus we call it 20xLB. Before we can use it, it needs to be diluted to a 1x concentration. If we needed 20 mL of the media, we would take 1 mL of the 20xLB buffer and diluted it with 19 mL of Dl water. To make enough buffer for this experiment, we need 50 mL for the gel...
Ascorbic Acid Concentration (g/L)
Volume of DCPIP in Full Syringe Used (mL)
Volume of DCPIP in Final Syringe (mL)
Total Volume of DCPIP Used (mL)
0.0
5 mL
4.9 mL
0.1 mL
0.50
5 mL
2 mL
3 mL
1.00
5 mL
1.2 mL
3.8 mL
1.50
5 mL
3.6 mL
6.4 mL
2.00
5 mL
2.8 mL
7.2 mL
Unknown Trial 1
5 mL
2.0 mL
3.0 mL
Unknown Trial 2
5 mL
2.2 mL
2.8 mL
Unknown Trial...
Lab 7 7. In this experiment, DCPIP was used to indirectly measure photosynthetic rate. If you were asked to design an experiment so that photosynthesis could be measured directly, what factors might you measure? 8. Compare your results for the absorption spectrum and the action spectrum. Explain any discrepancies between the wavelengths of light that are absorbed maximally and those wavelengths that cause the greatest photosynthetic activity. (Note that the wavelengths of light transmitted by each coloured filter can be...
i need help with number 8 and 9 please!
297 Experiment 24 10. Common ion effect a. Concentration of KHT final solution (50 mL) b. Volume of solution c. Mass of KCI d. Concentration of KCI 5.0x10 mL 271 381 f. Total [H [KHned g. Saturation temperature (C) h. Saturation temperature (K) 516 332.7S 0428 j. Why did the temperature have to be increased to dissolve the KHT when the KCI was added? B. K, by titration. 10.1:C 1. Temperature...
Part I: You are instructed to produce solutions of H2S04 and NaOH for your lab experiment. The concentration you should produce is shown in the table below. Complete the table and copy it into your lab notebook for use during your lab. You will be provided with a 3.0 M stock solution of H2SO4 and solid NaOH. You will need to make 100 mL of each solution. H2SO4 Compound NaOH 0.9 Solution Concentration (M) 1.12 Moles in 100 mL 112...
Prepare the buffer. In this lab we store our buffer at a concentration 20 times the concentration used in the experiment. Thus we call it 20xLB. Before we can use it, it needs to be diluted to a 1x concentration. If we needed 20 mL of the media, we would take 1 mL of the 20xLB buffer and diluted it with 19 mL of Dl water. To make enough buffer for this experiment, we need 50 mL for the gel...
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