Question

Which of the following would alter protein primary structure? Boiling A reducing agent like beta-mercaptoethanol (BME)...

Which of the following would alter protein primary structure?

Boiling

A reducing agent like beta-mercaptoethanol (BME)

decreasing pH

None of the above

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Answer #1

None of the above.

Explanation: all thses treatments disrupt the quaternary and tertiary structure of protein. For example beta mercaptaethanol breaks the sulphide bridge between amino acid, sulphide bond stable the quaternary and tertiary structure.  

Primary structure is the amino acid sequence joined by peptide bond. None of the above can disrupt the primary structure.

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