Before you perform any protein assays on an sample of unknown protein concentration, how can you...
Before you perform any protein assays on an sample of unknown protein concentration, how can you figure out the protein concentration range? Presumably, Bradford and Lowry assays work on certain protein ranges. If you had a sample of unknown concentration how would you determine its concentration range? Step by step if you could
Homework Answers
By only looking at the protein sample, we cannot determine if the concentration of unknown proteins is less or more than the protein standard.
First, we need to compare the OD of the unknown protein sample and the standard protein sample. If the OD of the unknown sample is more than the standard solution, then we need to dilute it so that it is less than the highest concentration of the standard solution. if the OD is less then we can proceed with the Bradford assay or Lowry assay.
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Before you perform any protein assays on an sample of unknown
protein concentration, how can you...
Could you please help me figure this out. This is based on the 'protein concentration quantification'...
Could you please help me figure this out. This is based on the 'protein concentration quantification' lab we conducted in class. We did both the Lowry and Bradford Reagent methods. There were 3 unknown protein concentrations (unknown A = Lactase; unknown B= Insulin; unknown C= BSA). We used 0.5 ml of each protein sample for our unknowns. Below are my unknown concentrations for the Lowry method: Unknown A = 225.3 ug/ml Unknown B = 238.1 ug/ml Unknown C= 517.5 ug/ml...
You have obtained 150 µL of a protein sample of which you want to perform a...
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A Bradford assay was carried out to measure protein concentration. The absorbance of five standard samples...
A Bradford assay was carried out to measure protein concentration. The absorbance of five standard samples and two unknown samples are listed in the table below. The Bradford reaction mixture (1 ml) was prepared as follows: 100 μl sample A or sample B + 200 μl Bradford reagent + 700μl 0.9% NaCl solution sample ID concentration (mM) absorbance (O.D.) std 1 100 0.78 std 2 50 0.4 std 3 25 0.18 std 4 12.5 0.08 std 5 0 0 ...
In this lab, you will quantitatively estimate the protein concentration in the following 3 unknown samples:...
In this lab, you will quantitatively estimate the protein concentration in the following 3 unknown samples: 1. Milk 2. Diet drink 3. High protein health food drink. Before you do the lab, you are expected to know the following: 1. How will you make the dilutions of the provided standard protein BSA (Bovine Serum Albumin) in the microfuge (1.7 ml small) tubes? Draw the chart for the same. 3 marks 2. How will you make the dilutions for the above...
6. If you have two proteins with unknown concentrations (4 points): -sample A: a protein that...
6. If you have two proteins with unknown concentrations (4 points): -sample A: a protein that contains polar uncharged amino acids, negatively charged amino acids and arginines, size: 40 KDa -sample B: a protein rich in lysines, size: 100 KDa A. If you want to separate both proteins, which acrylamide gel concentration would you chose? B. Which staining method (s) would you chose for this gel? Why?
you assay an unknown sample and its concentration falls below the linear range of your standard...
you assay an unknown sample and its concentration falls below the linear range of your standard curve. what can you do to solve this problem?
You need to neutralize a solution of an unknown concentration of sodium hydroxide before you can...
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4. (a) Determine the experimental concentration of protein in the unknown solution in mg/mL using the...
4. (a) Determine the experimental concentration of protein in the unknown solution in mg/mL using the Part II Data Table and the equation on page 2 for the absorbance assay at 280 nm. Hint: Convert the molar concentration (M or moles/liter) of BSA from absorbance assay at 280 nm method to mg/mL, assume that the molecular weight of BSA = 66.5 kDa [7 points 6500 g/mol Post-lab 10 Report Form: Determination the Concentration of a Protein You must show your...
20% Multi-Step Synthesis Sa) Show how you could perform the following synthesis. You may use any reagents that you need. 20% Multi-Step Synthesis Sa) Show how you could perform the following...
20% Multi-Step Synthesis Sa) Show how you could perform the following synthesis. You may use any reagents that you need.
20% Multi-Step Synthesis Sa) Show how you could perform the following synthesis. You may use any reagents that you need.
ncetoi paper) 1. BSA stock solution A has a concentration of 3.50 mg/mL. 1.00 mL of...
ncetoi paper) 1. BSA stock solution A has a concentration of 3.50 mg/mL. 1.00 mL of stock solution A is diluted to a final volume of 5.00 mL to prepare solution B. 1.00 mL of solution B is diluted to a final volume of 5.00 mL to prepare solution C. 1.00 mL of solution C is diluted to a final volume of 10.00 mL to prepare solution D. Calculate the concentrations of solutions E, C, and D. Show your work....
In this lab, you will quantitatively estimate the protein concentration in the following 3 unknown samples: 1. Milk 2. Diet drink 3. High protein health food drink. Before you do the lab, you are expected to know the following: 1. How will you make the dilutions of the provided standard protein BSA (Bovine Serum Albumin) in the microfuge (1.7 ml small) tubes? Draw the chart for the same. 3 marks 2. How will you make the dilutions for the above...
6. If you have two proteins with unknown concentrations (4 points): -sample A: a protein that contains polar uncharged amino acids, negatively charged amino acids and arginines, size: 40 KDa -sample B: a protein rich in lysines, size: 100 KDa A. If you want to separate both proteins, which acrylamide gel concentration would you chose? B. Which staining method (s) would you chose for this gel? Why?
4. (a) Determine the experimental concentration of protein in the unknown solution in mg/mL using the Part II Data Table and the equation on page 2 for the absorbance assay at 280 nm. Hint: Convert the molar concentration (M or moles/liter) of BSA from absorbance assay at 280 nm method to mg/mL, assume that the molecular weight of BSA = 66.5 kDa [7 points 6500 g/mol Post-lab 10 Report Form: Determination the Concentration of a Protein You must show your...
20% Multi-Step Synthesis Sa) Show how you could perform the following synthesis. You may use any reagents that you need.
20% Multi-Step Synthesis Sa) Show how you could perform the following synthesis. You may use any reagents that you need.
ncetoi paper) 1. BSA stock solution A has a concentration of 3.50 mg/mL. 1.00 mL of stock solution A is diluted to a final volume of 5.00 mL to prepare solution B. 1.00 mL of solution B is diluted to a final volume of 5.00 mL to prepare solution C. 1.00 mL of solution C is diluted to a final volume of 10.00 mL to prepare solution D. Calculate the concentrations of solutions E, C, and D. Show your work....
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