I perform PCR and obtain very little of the desired product. What can I do in...

Question

Question

I perform PCR and obtain very little of the desired product. What can I do in an attempt to increase the amount of PCR product that I obtain?

science biology

Answer 1

Answer #1

Answer 2

Similar Homework Help Questions

Identification of unknown Bacteria by sequencing rDNA Having a little trouble understanding the PCR process. This...

Identification of unknown Bacteria by sequencing rDNA Having a little trouble understanding the PCR process. This is a lab we did and some homework questions regarding the sequencing rDNA to find unknown bacteria. I hope by answering these I can have better understanding of the process. The more descriptive the better. Thank you! 2. A. List the reagents used in preparing master mix for PCR and write one sentence about why each one was necessary. B. Now that you have...
What is the difference between regular PCR and RT-PCR? In RT-PCR you can measure the amount...

What is the difference between regular PCR and RT-PCR? In RT-PCR you can measure the amount of DNA copied by adding a fluorescent dye. The fluorescence increases as the desired sequence is amplified. O In PCR, there is a higher chance of contamination with undesired samples because primers are less specific than in RT-PCR In RT-PCR, new sequences can be added each cycle which makes the process more dynamic and efficient. In PCR, the temperatures vary more than in RT-PCR...
What does the enzyme exonuclease I do? Why did you treat your initial PCR product with...

What does the enzyme exonuclease I do? Why did you treat your initial PCR product with exonuclease I before using the DNA as a template for Nested PCR? What would have been the result if you had not done an exonuclease I treatment?

Identification of unknown Bacteria by sequencing rDNA Having a little trouble understanding the PCR process. This...

Identification of unknown Bacteria by sequencing rDNA Having a little trouble understanding the PCR process. This is a lab we did and some homework questions regarding the sequencing rDNA to find unknown bacteria. I hope by answering these I can have better understanding of the process. The more descriptive the better. Thank you! 1. A. Name the gene that we will be sequencing in order to identify the unknown. Explain why this region is considered to be the target for...
What would have been needed in the PCR reaction to obtain bands for the gene that...

What would have been needed in the PCR reaction to obtain bands for the gene that was inserted into the vector? Give 3 examples of what CRISPR can be used to do. States)
When you perform a pcr reaction, what length of product is predicted? a. 5’ end one...

When you perform a pcr reaction, what length of product is predicted? a. 5’ end one primer to 5’ end of other b. 5’ end one primer to 3’ end of other c. 3’ end one primer to 3’ end of other d. not possible to predict the length

What can a business that has too little working capital do to increase it? · Decrease...

What can a business that has too little working capital do to increase it? · Decrease inventory · Increase short-term liabilities · Reduce current assets · Increase cash on hand
If i perform a Welch test to obtain the degrees of freedom, and i get 9.998984932...

If i perform a Welch test to obtain the degrees of freedom, and i get 9.998984932 as the answer, do i use 9 or 10 for the degrees of freedom? it usually is the lower number but this is so close to 10? or do we still have to use 9?
Add SYBR green to the remaining half of each PCR reaction product and perform a melting...

Add SYBR green to the remaining half of each PCR reaction product and perform a melting curve analysis. slowly increase the temperature of the samples from 60oC to 95oC, recording the fluorescence emitted from each sample with every 5oC increase in temperature. Draw the fluorescence (y axis) versus temperature (x axis) plot that you would expect to see for each product (1 mark each). Specify relevant temperatures for each sample on the x axes of your plots (1 mark each)...

D. After you run your PCR reaction, what next steps would you perform to determine that...

D. After you run your PCR reaction, what next steps would you perform to determine that the PCR amplified the region of interest? E. After your procedures in step D above, you decide to measure the concentration of DNA in your PCR product. Briefly explain how you would do this given the tools we have available. What values would you expect to confirm that you have a good DNA concentration and purity? F. Write and briefly describe a program you...