Question

Read thoroughly. View the two responses and answer the question below. Basically making a comparison and stating similarities. Reflect on the Wright et al. (2014) paper. Did you and your classmates tend to make the same mistakes or different errors from the students that Wright interviewed? Please reference an aspect of the Wright paper in your replies. Discuss whether you and your peers, made some of the same errors or different ones when making your descriptions.

Response 1:

DNA replication is a biological process of producing two identical replicas of DNA from one original DNA molecule. This process is described as semi-conservative because each DNA molecule is made up of one old strand of DNA and a new one. DNA replication occurs in all living organisms acting as the basis for biological inheritance. The first step in DNA replication is a separation of the two strands. This unzipping is done by an enzyme called helicase and results in the formation of a replication. Each separated strand acts as a template for creating a new strand of DNA. An enzyme called primase starts the process and makes a small piece of RNA called primer which marks the starting point for the construction of a new strand of DNA. After a starting point for the construction of a new strand of DNA is complete, an enzyme called DNA polymerase binds to the primer and starts to make a new strand of DNA. The DNA polymerase can only add DNA bases in one direction, from the 5’ end to the 3’ end. One of the new strands of DNA, the leading strand is made continuously in the 5’ to 3’ direction. The other strand, the lagging strand, runs in the opposite direction than the leading strand, therefore, it can’t be made in this continuous way. Due to the difference in the direction, the DNA polymerase can only make this strand in a series of small sections called Okazaki Fragments. After each fragment is started with an RNA primer, DNA polymerase adds a short row of DNA bases in the 5’ to 3’ end - this process is repeated. After a new DNA is made, the enzyme called exonuclease removes all the RNA primers from both strands of DNA and another DNA polymerase enzyme fills in the gaps that are left behind with DNA. Finally, the enzyme DNA ligase seals up the fragments of DNA in both strands to form a continuous double strand.

Response 2:

Replication is the method of constructing new copies of DNA from existing DNA strands. It happens within the nucleus and is the semiconservative method which implies that every new DNA molecule has one parent strand and one newly shaped strand. DNA synthesis starts at ORI (origin of replication) that are the AT bases rich sequences and terminates at the terminator. A DNA sequence with ORI and terminator makes a replicon that's capable of its replication. Helicase enzymes unwind the spiral of DNA using the energy of ATP. The single-stranded binding proteins then bind to the only strands of DNA to stop the rewinding. The ribonucleic acid primase enzyme makes primer, the short RNA strands that are elongated by DNA polymerases. DNA polymerases can't begin the formation of the latest DNA strands de novo and need a primer. The elongation of primer happens in 5' to 3' direction solely because the new nucleotide is added to free 3' OH of existing primer. The lagging strand is made within the direction opposite to the growth of replication fork in a semi discontinuous manner to permit the synthesis in 5' to 3' direction solely. The ribonucleic acid primers are removed by the exonuclease activity of DNA enzyme and therefore the DNA ligase enzymes join Okazaki fragment of the lagging strand along.

Answer 1