Describe one method which would allow you to demonstrate the presence of a specific gene in your cells.
Describe one method which would allow you to demonstrate the presence of a specific gene in...
Create a labeled diagram and describe the gene transfer process by which the Shiga toxin gene was picked up fromShigella dysenteriae and transferred to Escherichia coli. Be as specific as possible in your description of this process. Meaning be sure to label your donor and recipient cells as the bacterial species given depending on how the genes are being transferred.
Answer please
E. coli expresses a specific gene in the presence of lactose. The expression of this gene is under control by the lac operon, a sequence of nucleotides in the DNA which control the lac Operon al DNA ) laci CAP) (o) laczlacY
Describe 3 specific characteristics of a pathogen that would allow it to cause chronic disease. explain how each characteristics would allow infection to be chronic rather than acute
Describe in one paragraph which gene you think is mutated and why, based on the information in Flybase. Include the name of the gene, its function and your rationale for why you believe it is mutated in your flies? (The gene dmel/hsf is mutated because the mutated alleles exhibits compromised thermotolerance)
Which of the following techniques would allow you to determine whether a gene is alternatively spliced? a) In situ hybridization b) Northern blot c) Southern blot d) genomic DNA sequencing I honestly need help with this; I first thought it was Northern blot, but I'm starting to second guess myself on that and genomic DNA sequencing. Any help would be appreciated.
In eukaryotic cells, there are specific transcription factors (activators) that regulate gene expression. Describe the organization of eukaryotic genes (i.e. promoters/enhancers) and how transcription factors are able to control gene expression and enzyme activity. a) Throughout the course we discussed the enzyme lactase and how it is differently regulated (and therefore expressed) in different human populations. Describe how this gene is differently expressed in different populations and when this/these change(s) are predicted to have taken place. b) Differential gene expression...
You are analyzing a protein that you believe affects endocytosis, but you are not sure which stage. You create cells with your gene of interest mutated in the genome, and a tet-off system driving a wild-type (WT) copy of your gene (tet-off allows a specific gene to be turned off by adding a tetracycline family drug). After confirming that the system works, you culture the cells with and without tetracycline and in the presence of very high levels of EGF....
7. What are the modification systems, which allow one gene three) to code for many proteins? (Name b.
You hypothesize that your favorite gene, bmd19, is expressed more in the presence of alcohol. Which methods could you use to address this question? A) microarray & RT-PCR B) Western blotting & traditional PCR C) Southern blotting &Western blotting D) RT-PCR & Southern blotting
Describe the Initial-Rate method. Include enough detail that would allow someone who has never heard of this method to reproduce your method to the extent of rate constant determination. Also, provide at least 2 assumptions relevant to the initial rate method that simplify interpretation of the data.