You hypothesize that your favorite gene, bmd19, is expressed more in the presence of alcohol. Which methods could you use to address this question?
A) microarray & RT-PCR
B) Western blotting & traditional PCR
C) Southern blotting &Western blotting
D) RT-PCR & Southern blotting
Ans:-
Expression of gene of interest is best described by following methods
A) microarray and RT-PCR
DNA microarray analysis is done with blood sample of patient for ethanol presence.
And RT-PCR is then used to confirm relative transcript levels Of those detected in Microarray .
You hypothesize that your favorite gene, bmd19, is expressed more in the presence of alcohol. Which...
QUESTION Which of the following would you use to find a particular sequence within a sample of DNA? O RT-PCR O Western blotting O Northern blotting O Southern blotting QUESTION 7 The Sanger method for DNA sequencing requires 。A. dideoxynucleotides O8. DNA nucleotides OC DNA polymerase O D.A&B O E. A, B, & C .
You hypothesize that a DNA sequence located 3.0 kb upstream of the promoter of a gene you are studying contains an enhancer. To test your hypothesis, you use recombinant DNA techniques to insert a DNA fragment containing this DNA sequence into a plasmid also containing a “reporter gene.” Note:A reporter gene encodes for a protein that you can detect when expressed. For example, you could use the lacZ gene that encodes for the enzyme β-Galactosidase as a reporter gene. As...
You want to transform your yeast cells with a plasmid encoding your favorite gene and keep this gene stably expressed in the transformants. Which of the following is NOT a reason your experiment might fail? A. You transformed a linearized plasmid lacking telomeric sequences. B. The centromeric sequence on the plasmid is from a different organism. C. You forgot to co-transform telomerase with your plasmid. D. The plasmid lacks a functional ARS sequence.
You hypothesize that expression of Gene X, a gene for which you know the complete sequence in the organism of interest, may be an important contributor to the development of a phenotype (trait) that you study (e.g. eye development). What approach(es) would you use to manipulate the expression pattern of the gene to test your hypothesis?
B. Based on lanes 4 and 3, which cell type has the more tubulin protein-NIH/3T3 cells? Co cells? About the same? Can you tell? 14. As a way of contrasting the results of various molecular methods, answer each of the following questions. Note that each of these questions asks you to think about two methods that might seem superficially to yield similar information, but to focus how the results are different. A) What information would you get from a northern...
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You have carried out a two-hybrid screen with your favorite gene
(which codes for your favorite protein YFP) and identified a gene
that codes for a protein we’ll call “A.” YFP is only 90 amino acids
long. You wish to identify which part of YFP binds to A. You
construct three deletion alleles of YFP that are fused Gal4 binding
domain, one contains the N-terminal 30 amino acids of YFP (1-30),
one the middle 30 amino acids (31-60) and one...
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Review of Concepts: Gene Identification and Gene Knockout In humans, Xeroderma Pigmentosis (XP) is characterized by hypersensitive to UV light. This light creates a very high amount DNA damage leading to increased cancer rates. You are very interested in identifying a gene that might be resulting in this UV induced DNA damage in people suffering from XP. Propose a research design that will...