You have just finished purifying the pLIC-EGFP plasmid from DH5α cells. You measure the absorbance of...
You have just finished purifying the pLIC-EGFP plasmid from DH5α cells. You measure the absorbance of the DNA at 260 and 280 nm and find that the ratio of 260/280 is 1.6. Is the DNA pure? What do you propose is the contaminant?
Homework Answers
NO the DNA is not pure. The DNA isn't pure since the ratio must be higher than 1.8 amino acid sequence of proteins might be anticipated to have a tremendous influence.
Add Answer to:
You have just finished purifying the pLIC-EGFP plasmid from DH5α
cells. You measure the absorbance of...
question number 7 purifying plasmid from your cells, you will set up a teaction to cut...
question number 7
purifying plasmid from your cells, you will set up a teaction to cut the plasmid DNA with the restriction enzyme Bgl Il. See the map below for the positions of the Bgl Il restriction enzyme sites on the plasmid. How many DNA fragments would you expect if your restriction enzyme digest was complete and your plasmid contains insert? Question 7 Not yet graded/ 1 pts After you electrophoresed the plasmid digest in Question 6, your gel showed...
One of the common methods to quantify DNA is to measure A260 (UV absorbance at 260...
One of the common methods to quantify DNA is to measure A260 (UV absorbance at 260 nm) of DNA solution using a spectrophotometer. You took 10 µl of your plasmid DNA preparation and dilute with 990 µl of H2O. The A260of this diluted solution was 0.30. Calculate the concentration of your plasmid DNA preparation (before the dilution). It has been established that 50 µg/ml of double-stranded DNA gives A260 = 1.0. My plasmid DNA preparation is _____________________ mg/ml. From this...
1. Why does the plasmid you are purifying have the beta-lactamase gene? 2. You are using...
1. Why does the plasmid you are purifying have the beta-lactamase gene? 2. You are using a small column to bind DNA molecules during purification of the plasmid DNA. This column binds DNA via ionic interactions. What is the charge on the column which allows it to bind DNA? Answer positive" or "negative and explain your answer. 3. Where is the plasmid at the END of each of the following steps? Circle the letter of the correct answers for each...
After purification of your favorite plasmid you use a spectrophotometer to find the concentration of your...
After purification of your favorite plasmid you use a
spectrophotometer to find the concentration of your DNA and observe
the graph below. Resketch by hand this graph on your answer page
with exact axis labels and scales. Redraw the red line. Draw, in
your estimation, the absorption spectrum of what is expected of a
good DNA prep.
OD o X Load your sample and press the measure button. Blank Re-Blank Sample ID: Pedestal ort pectra ple volume Type: DNA 50.00...
You have a solution that is a mixture of two compounds, A and B. The molar...
You have a solution that is a mixture of two compounds, A and B. The molar extinction coefficient (e) of substance A at 260nm and 280nm is 5248 and 3150 respectively. Compound B has a molar extinction coefficient of 311 and 350 at 260 nm and 280 nm rspectively. You determine that the solution has an absorbance of 0.83 at 260 nm and 0.67 at 280 nm. Can the individual concentrations of A and B be calculated from these data?...
DNA quantitation. You measure the absorption spectrum for a sample of DNA and obtain the values...
DNA quantitation. You measure the absorption spectrum for a sample of DNA and obtain the values listed below. The DNA had been diluted 10 fold. Remember that a solution of DNA that is 50μg/ml (or 50ng/μl) has an absorbance of 1.0 at a wavelength of 260 nm. What is the concentration of the DNA in this sample (In this question DO NOT ignore the problem of dust in the sample and give answer in ng/ul). A260 = 0.80 A280...
Q5: (2) DNA quantitation. You measure the absorption spectrum for a sample of DNA and obtain...
Q5: (2) DNA quantitation. You measure the absorption spectrum for a sample of DNA and obtain the values listed below. The DNA had been diluted 10 fold. Remember that a solution of DNA that is 50μg/ml (or 50ng/μl) has an absorbance of 1.0 at a wavelength of 260 nm. What is the concentration of the DNA in this sample (In this question DO NOT ignore the problem of dust in the sample and give answer in ng/ul). A260 = 0.80...
7. You have performed a cloning experiment in which you digested genomic DNA from starfish cells...
7. You have performed a cloning experiment in which you digested genomic DNA from starfish cells with an enzyme and ligated the fragments into plasmid vectors digested with the same enzyme. The plasmid you are using contains a gene for ampicillin resistance and an MCS located within the Lacz gene. For the ligation, you added three times as much of the digested plasmid as the insert. After introducing the ligated DNA into cells, you observe 200 blue colonies and 33...
You have prepared some plasmid DNA in lab and need to know its concentration so you...
You have prepared some plasmid DNA in lab and need to know its concentration so you can load 1 ug on a gel. You dilute 1 uL with 99 uL of water and the resulting sample has an absorbance of 0.075. What volume of the original sample do you need to load onto the gel? (Show math for partial credit and make sure you are answering the question I am actually asking.)
1. You have generated the following standard curve after a Bradford Assay to measure Absorbance vs...
1. You have generated the following standard curve after a Bradford Assay to measure Absorbance vs samples of known BSA concentrations. Now you need to determine the amount of protein in two mitochondria samples taken from fish, one taken from fish under normal conditions and the other taken from fish under oxidative stress. The absorbance reading for the normal fish fraction was A595=175, and the absorbance reading for the stressed fish fraction was A595= 275 Bradford Assay Standard Curve y...
question number 7
purifying plasmid from your cells, you will set up a teaction to cut the plasmid DNA with the restriction enzyme Bgl Il. See the map below for the positions of the Bgl Il restriction enzyme sites on the plasmid. How many DNA fragments would you expect if your restriction enzyme digest was complete and your plasmid contains insert? Question 7 Not yet graded/ 1 pts After you electrophoresed the plasmid digest in Question 6, your gel showed...
1. Why does the plasmid you are purifying have the beta-lactamase gene? 2. You are using a small column to bind DNA molecules during purification of the plasmid DNA. This column binds DNA via ionic interactions. What is the charge on the column which allows it to bind DNA? Answer positive" or "negative and explain your answer. 3. Where is the plasmid at the END of each of the following steps? Circle the letter of the correct answers for each...
After purification of your favorite plasmid you use a
spectrophotometer to find the concentration of your DNA and observe
the graph below. Resketch by hand this graph on your answer page
with exact axis labels and scales. Redraw the red line. Draw, in
your estimation, the absorption spectrum of what is expected of a
good DNA prep.
OD o X Load your sample and press the measure button. Blank Re-Blank Sample ID: Pedestal ort pectra ple volume Type: DNA 50.00...
7. You have performed a cloning experiment in which you digested genomic DNA from starfish cells with an enzyme and ligated the fragments into plasmid vectors digested with the same enzyme. The plasmid you are using contains a gene for ampicillin resistance and an MCS located within the Lacz gene. For the ligation, you added three times as much of the digested plasmid as the insert. After introducing the ligated DNA into cells, you observe 200 blue colonies and 33...
1. You have generated the following standard curve after a Bradford Assay to measure Absorbance vs samples of known BSA concentrations. Now you need to determine the amount of protein in two mitochondria samples taken from fish, one taken from fish under normal conditions and the other taken from fish under oxidative stress. The absorbance reading for the normal fish fraction was A595=175, and the absorbance reading for the stressed fish fraction was A595= 275 Bradford Assay Standard Curve y...
Most questions answered within 3 hours.
-
Where is the error in this code sequence?
String s1 = "Hello";
String s2 = "ello";...
asked 10 months ago -
Financial data for Joel de Paris, Inc., for last year
follow:
Joel de Paris, Inc.
Balance...
asked 10 months ago -
Consider this reaction:
Al2(SO4)3 (aq)+ BaCl3
(aq) Al2Cl6 (aq)- +
3BaSO4(s) . What is the...
asked 10 months ago -
Suppose that Savneet is considering increasing her
recent random sample from 20 car rentals to 40...
asked 10 months ago -
Trucks arrive at an unloading terminal at an average rate of 120
per hour.
Trucks arrive...
asked 10 months ago -
Why are methanol and ethanol completely soluble in water while
octanol is not very little soluble....
asked 10 months ago -
A facilities manager at a university reads in a research report
that the mean amount of...
asked 10 months ago -
When the CuSO4 is rehydrated by adding water to the anhydrous
compound, is this an endothermic...
asked 10 months ago -
A ray of sunlight is passing from diamond into crown glass; the
angle of incidence is...
asked 10 months ago -
A block of mass 0.249 kg is placed on top of a light, vertical
spring of...
asked 10 months ago -
how do the kidneys compensate in the presences of acidosis
a) trigger hyperventilate
b) reserve acid...
asked 10 months ago -
Question 501 pts
The rental rate of capital to the firm increases. Which of the
following...
asked 10 months ago