Question

Which of the following techniques could be used to accurately and precisely quantify gene expression? Select all that apply.

Question 6 options:

	DNA microarray
	RNA - Sequencing
	qRT-PCR
	RT-PCR
	RNA in situ hybridization

Answer 1

DNA microarray

RT – PCR

RNA in situ hybridization

These are the methods or techniques which are used for accurately profiling and finding out about gene sequence. With help of DNA microarray, it helps in measuring and sequencing the level of genes in large simultaneously in one genomic sequence and it can help in quantifying multiple regions within a single genome. The RT-PCR method is another sequencing method majorly used in research labs and it helps in studying and sequencing gene expression, majorly helps in differentiating the exons from the introns. Through this method it also helps in finding out about many genetic disorders. The RNA in situ hydridization is a method which is used for accurate profiling and sequencing of genes and DNA with help of labeled complementary RNA, DNA and probes which bind to specific RNA or DNA sequence within the gene.