Question

1.

cDNA has the same sequence as its template i) ________ but consists of ii) ________.

Question 1 options:

	i) mRNA; ii) dNTPs
	i) mRNA; ii) ddNTPs
	i) DNA; ii) dNTPs
	i) DNA; ii) ddNTPs
	i) mRNA; ii) rNTPs

2.

Which of the following is an accurate description of the probes used in DNA microarrays?

Question 2 options:

	cDNA copies of fragmented RNA isolated from samples cells
	More than one of these responses is correct
	Radiolabeled single stranded DNA fragments used to visualize complementary DNA bound to a solid surface
	Specific DNA fragments bound in an ordered pattern to a microchip
	Specific DNA fragments labeled with a fluorophore and bound in an ordered pattern to a microchip

3.

Quantitative Reverse Transcription PCR (qRT - PCR) is ultimately used to:

Question 3 options:

	produce an mRNA copy of DNA, which is then amplified and quantified.
	quantify the amount of target DNA in the original sample.
	More than one of these responses is correct.
	amplify and quantify target mRNA in the original sample.
	quantify the protein content in a particular sample.

Answer 1

1. Answer: option 1.

• In DNA, 5’ to 3’ strand of DNA serve as coding strand. 3’ to 5’ strand of DNA act as template strand.
• RNA is synthesized by complementary base pairing with the template strand.
• Thus, the RNA will have same sequence as coding strand (only T is replaced by U) and is oriented in 5’ to 3’ direction.
• cDNA is converted to mRNA with the enzyme reverse transcriptase .
• Thus, it has same sequence as the mRNA and has dNTPs as it is DNA.

2. Answer: option 2

• The hybridization technique with probe is used to identify samples.
• RNA or DNA probes may be used.
• Probes are radiolabeled, or fluorescent tagged is specifically selected for the gene of interest.
• The probes hybridize by formation of hydrogen bond with the desired sequence of gene of interest.

3. Answer: option 1

• In qRT-PCR, the starting sample is RNA.
• RNA is first converted to cDNA by reverse transcription process with the help of enzyme reverse transcriptase.
• The DNA thus generated is amplified by Process of quantitative PCR.