How many CFUs are in the original sample with a dilution of 1/100,000, and 63 colonies.
The formula of CFU count in 1ml of original sample is :
= CFU in diluted sample / Dilution factor
So, As dilution factor is given as 1/100,000, and CFU in diluted sample is given as 63 colonies.
therefore, CFU count in original sample = 63/(1/100,000)
= 63 x 100,000
= 63 x 104 CFU/ml
How many CFUs are in the original sample with a dilution of 1/100,000, and 63 colonies.
6) How many bacteria would be in 1 ml of the original sample if the 108 dilution has 200 colonies? 7) What criteria must your plates meet in order to be considered "countable" ? How many bacteria per milliliter of sample would be "acceptable" to the FDA? - 86 -
How many microliters of original sample are required to produce a final dilution of 10-3 in a total volume of 1,000 mL? • 1 microliter is 10-6 L or 10-3 mL. • The dilution is equal to M2/M1
1)How many microliters of original sample are required to produce a final dilution of 10-2 in a total volume of 5 mL? 1 microliter is 10-6 L or 10-3 mL. 2)A 0.00001 dilution is performed on a culture of bacteria in order to perform viable plate counts. From the dilution, *0.1 mL* of solution is plated on solid media, and 197 colony forming units grow on the plate. How many bacteria are in a single mL of the original culture?...
you count 187 CFUS on a spread plate. The plate was prepared by spreading 0.2ml of a 1:100 dilution of a 1:50 dilution of a 1:10 dilution of the original sample. What is the concentration of the original sample. What is the concentration of the original culture in cells/ml
The following details the results of a serial dilution of E. coli: Effective Dilution Factor on Plate CFUs 105 1095 106 178 107 5 108 None The "effective dilution factor on plate" already takes into account the fact that you plated 0.1 ml of the dilution. How many CFUs/ml were present in the original sample? a. 1.78 x 10^8 b. 1.14 x 10^8 c. 1.125 x 10^8 d. 1.095 x 10^8
0.1 ml of a 10-4 dilution of M. luteus was plated. After incubation, 80 colonies were counted. How many colony-forming units per ml were in the original E. coli sample?
What is the original concentration of cells in a sample that produced 218 colonies after spread - plating 0.1 ml from a tube with a 5/100000 overall dilution factor?
1. You have been given a sample that is a 1/50 dilution of the original. You measure 3.4 mg glucose in 0.5 ml of that sample. What is the concentration in the original sample? If you have 400 ml of that sample, how many grams of glucose is in the total sample?
.Viable plate count Using the formula: cfu/ml = (no. of colonies x dilution factor) / volume of culture plate In plate 10-4 there were 150 colonies counted. Experimental process: Pipet 0.25 mL of the S. cerevisiae solution into the test tube labeled 10-1. Pipet the solution up and down several times to ensure all organisms are rinsed from the pipet into the test tube. Pipet 0.25 mL of the 10-1 S. cerevisiae solution into the test tube labeled 10-2. Pipet...
Two parallel dilution series were made from the same original sample. The plates with sample volumes of 10- mL from e ach dilution series yielded 144 and 93 colonies. Suggest reasonable sources of error. What are the only circumstances that would correctly produce countable plates from two different dilutions?