Question

Name all possible unwanted products of the ligation reaction you will be performing in the lab. The reaction will involve incubating blunt-ended plasmid and blunt-ended PCR product with DNA ligase and ATP.Name all possible unwanted products of the ligation reaction you will be performing in the lab. The reaction will involve incubating blunt-ended plasmid and blunt-ended PCR product with DNA ligase and ATP.

-primer dimers  

-self-ligation of inserts  

-ligation of insert with primers  

-ligation of primers with vector  

-ligation of vector with 2 inserts  

-self-ligation of vector

Answer 1

Primer dimers are formed by self ligation of primers .

Self ligation of insert can also occur.

Ligation of insert with primers .

Ligation of primers with vector.

Ligation of vector with 2 inserts .

Self ligation of vector .

Ligation of primers with insert .