Suppose you construct a BSA protein standard curve (y-intercept = 0) and find that a point...
Suppose you construct a BSA protein standard curve (y-intercept = 0) and find that a point on the line is 0.347 A 595/20 μg. If an experimental tube containing 7 μl of a 10 -1 dilution of unknown solution gives an A 595 of 0.201, what is the protein concentration of the original unknown solution?
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Suppose you construct a BSA protein standard curve (y-intercept
= 0) and find that a point...
2.(1 point) You have 125 μLof a protein sample. Of this, you dilute30μLinto 120μLof buffer. 50μL...
2.(1 point) You have 125 μLof a protein sample. Of this, you dilute30μLinto 120μLof buffer. 50μL of that dilution were used for a Bradford assay and produced an absorbance of 0.53.Using the standard curveyou built in the lab using BSA, find the protein concentration (in μg/μL) of the original protein sample. Show your work. (Hint: first find the dilution fold of the sample that was used for the assay.) Note: equation from standard curve: y=0.0093x + 0.1877 show all work.
1. You want to prepare a 1:50 dilution of your protein extract in a total volume...
1. You want to prepare a 1:50 dilution of your protein extract in a total volume of 1000 uL. You will need ___ uL of protein extract and ___ uL of water. 2. You are provided with a solution of BSA that is 100 ug/mL and make a 10-2 dilution? What is the resulting concentration of the DILUTED BSA? 3. Which of the following statements about enzymes is true? Select one: a. Enzymes increase the rate of a chemical reaction...
In lab, you diluted your unknown protein solution by combining 14 ?L of unknown protein solution...
In lab, you diluted your unknown protein solution by combining
14 ?L of unknown protein solution with enough
PBS buffer to obtain a total volume of 100 ?L, then added 1000 ?L Bradford reagent. After waiting ten
minutes, the absorbance at 595 nm was 0.206. Using the standard
curve from the previous question, calculate the total concentration
of protein (in ?gmL) in the original sample.
Slope = 0.02371 Intercept = 0.01132 r2
= 0.973
A Bradford assay was carried out to measure protein concentration. The absorbance of five standard samples...
A Bradford assay was carried out to measure protein concentration. The absorbance of five standard samples and two unknown samples are listed in the table below. The Bradford reaction mixture (1 ml) was prepared as follows: 100 μl sample A or sample B + 200 μl Bradford reagent + 700μl 0.9% NaCl solution sample ID concentration (mM) absorbance (O.D.) std 1 100 0.78 std 2 50 0.4 std 3 25 0.18 std 4 12.5 0.08 std 5 0 0 ...
please help with ALL questions thank you 1. Using excel, plot your standard curve based on...
please help with ALL questions thank you
1. Using excel, plot your standard curve based on Table 9-1 and calculate the line of best fit. Put concentration in the x column and OD in the y to solve for the concentrations of your unknown in Table 9-3. Report the slope and the y-intercept below. (1 mark) Slope (m) = v-intercept(b) = 2. Can this standard curve for yeast cells be used for cali cells (why/ why not?) (2 marks) 3....
INTRODUCTION Albumin is synthesised in the liver at a rate that is dependent on dietary protein...
INTRODUCTION Albumin is synthesised in the liver at a rate that is dependent on dietary protein intake. Little is lost from the body by excretion. It is catabolized in various tissues, where it is taken up by cells by pinocytosis. Albumin has a vast capacity for ligand binding due to the large number of charges on each molecule as well as the very large number of molecules available. This binding property of albumin is related to one of its functions,...
INTRODUCTION Albumin is synthesised in the liver at a rate that is dependent on dietary protein...
INTRODUCTION Albumin is synthesised in the liver at a rate that is dependent on dietary protein intake. Little is lost from the body by excretion. It is catabolized in various tissues, where it is taken up by cells by pinocytosis. Albumin has a vast capacity for ligand binding due to the large number of charges on each molecule as well as the very large number of molecules available. This binding property of albumin is related to one of its functions,...
1. What is the definition of an 'equivalence point' in an acid/base titration? (1 point) 2....
1. What is the definition of an 'equivalence point' in an acid/base titration? (1 point) 2. In part one of the experiment, you will prepare the acid solutions being titrated from a stock solution. Describe how you will accurately prepare 10.00 mL of 0.100 M HCl solution using a 1.00 M HCl stock solution. In your response to this question, be very specific about the quantities of stock solution and deionized water to be used in the dilution and the...
Below is data that a researcher collected on the observed decay of a chemical in water...
Below is data that a researcher collected on the observed decay of a chemical in water as a function of time. The Researcher used an analytically technique to quantify the chemical that reports relative “peak area” or “response” values. As a result, the researcher analyzed a series of known “standards” of chemical “A” to construct a standard curve, from which the concentrations of “A” in the experimental samples could be calculated. It is known that the chemical decays by a...
lab question 1. What is the basis of the different purification methods? 2. What are some...
lab question 1. What is the basis of the different purification methods? 2. What are some of the factors the might have interfered with your results? 3. How might you improve the process to increase the yield and purity? lab process E. coli BL21 (DE3) cells were transformed with the pET Topo-1521 vector containing a reading frame encoding the green fluorescent protein (GFP). Cells were cultured in M9ZB media at 37°C until the absorbance at 600 nm reached 0.7, at...
In lab, you diluted your unknown protein solution by combining
14 ?L of unknown protein solution with enough
PBS buffer to obtain a total volume of 100 ?L, then added 1000 ?L Bradford reagent. After waiting ten
minutes, the absorbance at 595 nm was 0.206. Using the standard
curve from the previous question, calculate the total concentration
of protein (in ?gmL) in the original sample.
Slope = 0.02371 Intercept = 0.01132 r2
= 0.973
please help with ALL questions thank you
1. Using excel, plot your standard curve based on Table 9-1 and calculate the line of best fit. Put concentration in the x column and OD in the y to solve for the concentrations of your unknown in Table 9-3. Report the slope and the y-intercept below. (1 mark) Slope (m) = v-intercept(b) = 2. Can this standard curve for yeast cells be used for cali cells (why/ why not?) (2 marks) 3....
1. What is the definition of an 'equivalence point' in an acid/base titration? (1 point) 2. In part one of the experiment, you will prepare the acid solutions being titrated from a stock solution. Describe how you will accurately prepare 10.00 mL of 0.100 M HCl solution using a 1.00 M HCl stock solution. In your response to this question, be very specific about the quantities of stock solution and deionized water to be used in the dilution and the...
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