Is purifying genomic DNA sufficient to determine the sequence of a SNP? Why or why not?
Is purifying genomic DNA sufficient to determine the sequence of a SNP? Why or why not?
Homework Answers
NO, DNA purification is not sufficient to determine the sequence of a SNP ( single nucleotide polymorphism ).
DNA purification is done to remove the substance or enzyme which act as an inhibitor in the further reaction process.
SNP refer to the single nucleotide change because of which the DNA of one individual differ from other. RFLP use restriction enzyme ,there are regions in the genome where restriction site is not present so, those change can be observed by SNP . SNP generally found in intronic regions.
DNA purification is followed by RT-PCR in some cases to determine the sequence . DNA purification does not provide any information regarding the sequence. In SNP we require DNA sequence information to determine the change.
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Is purifying genomic DNA sufficient to determine the sequence of
a SNP? Why or why not?
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