Question

i) Which statement about the biosynthesis of proteins is NOT correct?

tRNA molecules function as carriers of the amino acids for protein biosynthesis.

The growing peptide chain results from a nucleophilic addition-elimination reaction by an amino group at an ester group.

A codon is a three-base sequence in mRNA that is specific for a particular amino acid.

The biosynthesis of proteins is called transcription.

ii) Which of the following does not occur during pyrosequencing?

A 3'-OH attacks the α-phosphorus of a 3'-protected 2'-deoxyribonucleotide triphosphate.

Enzymes are used that are specific for cleaving a particular 3'-base.

Enzymes cause light to be emitted when pyrophosphate is detected.

The base sequence is determined by synthesis.

iii) Which of the five heterocyclic bases contains a methyl group?

cytosine

thymine

guanine

uracil

adenine

iv) Assume that all OH groups of the polyphosphoric acid in ATP are deprotonated. What is the total charge of ATP?

0

−1

−2

−3

−4

v) The nucleophilic attack on ATP may involve a variety of leaving groups. Which group is cleaved off if the nucleophile attacks the α P-atom?

(O-)2PO-O-PO(O-)2

(O-)2PO-adenosine

(O-)2PO-O-P(O-)O-adenosine

(O-)2PO-O-P(O-)O-O-P(O-)O-adenosine

vi) The number of A-C in double-stranded DNA is equal to the number of _____.

G-T

A-G

C-G

T-G

G-C

Answer 1

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