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Gorter and Grendel isolated 4.74X109 erythrocytes, dissolved these in benzene and made a monolayer as per...

Gorter and Grendel isolated 4.74X109 erythrocytes, dissolved these in benzene and made a monolayer as per Langmuir experiment. Surface area of the monolayer was measured to be 0.89x 1012 µm2. Surface area of one erythrocyte is 100µm2. What can be interpreted from this data? Is erythrocyte membrane a bilayer or monolayer?

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Answer #1

Gorter and Grendel predicted quite simply that if a plasma membrane were really a bilayer then its surface area should be half that occupied by all its amphipathic lipids spread out in a monolayer.

4.74X109 x100 = 474 x 1012

The result produced a cell with monolayer surface area ratio of approximately 1:2 for a number of different mammalian red cells, thereby confirming their bilayer model of the plasma membrane.

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