Question

Addition of ________causes the induction of the ______________ polymerase. This polymerase then binds to the T7 ________________ upstream of the rGFP gene, resulting in the _____________________ of rGFP gene to produce rGFP mRNA. This mRNA is then ______________________ to produce the rGFP protein.

I think it goes IPTG, T17 RNA, RNA. I do not know if this is right and how to fill the rest of the spaces. Please help!

Answer 1

Addition of HEAT causes the induction of the T7 RNA polymerase. This polymerase then binds to the T7 RNA POLYMERASE PROMOTER upstream of the rGFP gene, resulting in the TRANSCRIPTION of rGFP gene to produce rGFP mRNA. This mRNA is then TRANSLATED to produce the rGFP protein.

T7 RNA polymerase is a heat-inducible enzyme that's activated within the presence of a heat source. This polymerase binds to a selected sequence referred to as the T7 RNA Polymerase promoter sequence. It moves along the DNA sequence leading to the synthesis of mRNA during a process referred to as transcription.
This mRNA is converted into a protein product through a process referred to as translation.