18. Draw the lac operon with all cis and trans acting factors A. in the presence...
18. Draw the lac operon with all cis and trans acting factors
A. in the presence of lactose
B. in the presence of lactose plus glucose
C. in the presence of lactose plus glucose in a OC mutant
Homework Answers
The answer of the question is given below if you have any doubt then feel free to ask in the comment box and guys don't forget to trigger the thumbs up.
A. in presence of lactose
B. In the presence of lactose plus glucose
C. In the presence of lactose plus glucose in a OC mutant
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18. Draw the lac operon with all cis and trans acting
factors
A. in the presence...
Catabolite repression can regulate the expression of the lac operon in E. coli. In terms of...
Catabolite repression can regulate the expression of the lac operon in E. coli. In terms of this positive regulation of the lac operon: What is the activator? Would it work in cis or trans? What molecule binds to the allosteric domain of the activator? What influences strongly the levels of the molecule you answered for part b? To what regulatory sequence does the activator bind? What part/domain of the activator? What is the effect of this binding? Name the two...
Briefly explain your answers to the following questions. A mutation (Mutation A) in the lac operon...
Briefly explain your answers to the following questions. A mutation (Mutation A) in the lac operon of E. coli leads to an inability to ferment lactose, and the expression of the operon is always off. Introduction into the mutant of an F' factor containing the wild type lac operon does NOT restore the ability to ferment lactose, i.e., there is no expression of the operon from the plasmid either. a. What is the probable nature of Mutation A? Is the...
Bacteria which are stable partial diploids (F’) for the lac operon are grown in the presence...
Bacteria which are stable partial diploids (F’) for the lac operon are grown in the presence or absence of lactose, as indicated below. The presence (+) or absence (-) of β-galactosidase and permease expression are investigated in these cultures. Which of the following options (A-E) would be the expected results for bacteria with the genotype IS P+ O+ Z+ Y- / I+ P+ OC Z– Y+ ? β-galactosidase Permease No lactose Lactose No lactose Lactose A - + - +...
The lac operon contains a DNA sequence known as the lac promoter (P or P+ for...
The lac operon contains a DNA sequence known as the lac promoter (P or P+ for wild type; P– for mutant (RNA polymerase does not bind)) that serves as the RNA polymerase binding site. The lac operon also contains a DNA sequence known as the Lac operator (O or O+ for wild type; O– or Oc for mutant (lac repressor cannot bind)) which is the binding site for lac repressor. The lac repressor, a protein, is encoded by the lac...
help pleaseee In the lac operon, positive control occurs through the presence of the CAMP-CAP complex....
help pleaseee
In the lac operon, positive control occurs through the presence of the CAMP-CAP complex. This complex is not available in the presence of glucose. this would mean that: When lactose is present, the lac operon will be activated only if glucose is absent When lactose is absent, the lac operon will be activated only if glucose is also present When lactose is present, the lac operon will not be activated regardless of glucose presence When lactose is present,...
5. Jacob, Monod, and Pardee used various E.coli mutants to help determine how the lac operon...
5. Jacob, Monod, and Pardee used various E.coli mutants to help determine how the lac operon is regulated. The descriptions of some of the mutants are listed. For each E. coli mutant determine if the lac operon would be on or off in a lactose-only environment. laci mutant: This mutant produces a super repressor that is not inactivated by allolactose. It will still stick. Operater will be off, lacO mutant: This mutant has an altered operator sequence so that the...
1. In the presence of glucose the lac operon is: 1. On 2. Off 3. Could...
1. In the presence of glucose the lac operon is: 1. On 2. Off 3. Could be either on or off 2.In the presence of lactose the lac operon is: a. On b. Off c. Could be either on or off 3. How do tetrahymena feed? a. Flagellar action b. Phagocytosis c. Absorption d. Exocytosis
Imagine you are carrying out research on the lac operon. You isolate six mutations in the...
Imagine you are carrying out research on the lac
operon. You isolate six mutations in the lac operon by
measuring the amount of beta-galactosidase made in mutant cell line
under three different conditions: no lactose/no glucose; lactose
only; and lactose/glucose. Your results are shown in the table.
Strain
No Lactose, No Glucose
Lactose
Lactose, Glucose
Wild-type
None
High
Low
Mutant 1
None
None
None
Mutant 2
None
None
None
Mutant 3
None
Low
Low
Mutant 4
None
Low
Low
Mutant...
help with this genetic problem You have a mutant E.coli with a lac operon (lacMuT) which...
help with this genetic problem
You have a mutant E.coli with a lac operon (lacMuT) which is not responding to lactose control You create an F episomal plasmid with the wild-type lac operon (lacW) and transform it into your mutant E. coli Genotype Mutant (lac) Mutant (lac Merizygote (lacMUT+ lacMUT)No Merizygote (lalacYes Lactose Added? Beta-galactosidase activity 100% 100% 100% 200% MUT Yes MUT MUT Is the mutation in the lacl trans-factor or the lacO cis-element? Describe the likely mutation and...
A lac operon mutant is grown in the presence and absence of lactose and tested for...
A lac operon mutant is grown in the presence and absence of lactose and tested for activity of the biosynthesis genes ‘Z’, ‘Y’, and ‘A’. Given the genotype below, which biosynthetic genes would be expressed in the presence of lactose? I+ P+ O+ Z+ Y- A+/ F’ I- P+ O+ Z+ Y- A+ Select one: a. none b. Z, Y, and A c. P and O d. Z and A e. I, P, O, Z, Y, and A
help pleaseee
In the lac operon, positive control occurs through the presence of the CAMP-CAP complex. This complex is not available in the presence of glucose. this would mean that: When lactose is present, the lac operon will be activated only if glucose is absent When lactose is absent, the lac operon will be activated only if glucose is also present When lactose is present, the lac operon will not be activated regardless of glucose presence When lactose is present,...
5. Jacob, Monod, and Pardee used various E.coli mutants to help determine how the lac operon is regulated. The descriptions of some of the mutants are listed. For each E. coli mutant determine if the lac operon would be on or off in a lactose-only environment. laci mutant: This mutant produces a super repressor that is not inactivated by allolactose. It will still stick. Operater will be off, lacO mutant: This mutant has an altered operator sequence so that the...
Imagine you are carrying out research on the lac
operon. You isolate six mutations in the lac operon by
measuring the amount of beta-galactosidase made in mutant cell line
under three different conditions: no lactose/no glucose; lactose
only; and lactose/glucose. Your results are shown in the table.
Strain
No Lactose, No Glucose
Lactose
Lactose, Glucose
Wild-type
None
High
Low
Mutant 1
None
None
None
Mutant 2
None
None
None
Mutant 3
None
Low
Low
Mutant 4
None
Low
Low
Mutant...
help with this genetic problem
You have a mutant E.coli with a lac operon (lacMuT) which is not responding to lactose control You create an F episomal plasmid with the wild-type lac operon (lacW) and transform it into your mutant E. coli Genotype Mutant (lac) Mutant (lac Merizygote (lacMUT+ lacMUT)No Merizygote (lalacYes Lactose Added? Beta-galactosidase activity 100% 100% 100% 200% MUT Yes MUT MUT Is the mutation in the lacl trans-factor or the lacO cis-element? Describe the likely mutation and...
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