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Which of the following oligonucleotides has the highest Tm when annealed to their complementary strands of...

Which of the following oligonucleotides has the highest Tm when annealed to their complementary strands of DNA?

A) GGAACCCAAGCGCTTAGC

B) GAACCAAGCTTTAGC

C) GGACCCAGCGCTAGC

D) ATTATTCAAATGGATTA

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Answer #1

GGAACCCAAGCGCTTAGC (option A) oligonucleotides has the highest Tm when annealed to their complementary strands of DNA

explanation :- because this oligonucleotide contain highest GC content and higher the number of GC bases highest the Tm.

melting temperature can be calculated by formula

Tm=4(G+C) + 2(A+T)

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