1.You are using the mutagen hydroxylamine which replaces a G·C base pair with an A·T base pair (G to A transition).
a. Could hydroxylamine be used to induce reversion of a nonsense mutation by directly altering the mutated codon? Briefly explain your reasoning and be sure to include all possible nonsense mutations.
b. Could hydroxylamine cause a phenylalanine codon to be mutated to atyrosine codon? Briefly explain your reasoning
c. Starting with each codon for glycine and allowing hydroxylamine to cause one substitution, which codons and corresponding amino acids (other than glycine) are possible as outcomes of hydroxylamine mutagenesis?
(a) nonsense mutations generate stop codons -UGA, UAG, UAA
Hydroxylamine shoes Gvto A mutation giving UAA in all the 3 stop codon cases, so it cannot reverse the nonsense mutation.
(b) Phe codon - UUU and UUC. Hydroxylamine cannot change it to Tyr because it only does G to A transition and there's no G in Phe codon.
(c) Glycine codons and their mutagenic outcomes:
GGU - AGU (Ser), GAU (Asp)
GGC - AGC(Ser), GAC (Asp)
GGA - AGA(Arg), GAA (Glu)
GGG - AGG(Arg), GAG(Glu)
1.You are using the mutagen hydroxylamine which replaces a G·C base pair with an A·T base...
1. You have used a mutagen to induce mutations in a DNA sequence. If the original DNA strand is 5'-ATGGGACTAGATACC-3', then which of the following represents a nonsense mutation? (1pt) 5'-ATGGGTCTAGATACC-3' 5'-ATGCGACTAGATACC-3' 5'-ATGTGACTAGATACC-3' 5'-ATGGGACTAAGATACC-3' 2. A mutation that changes a codon sequence, and subsequently changes the amino acid that should have been placed at that point in the polypeptide chain, is called a… (1pt) silent mutation frameshift mutation missense mutation nonsense mutation 3. Excision repair corrects DNA by (1pt) correcting A=T...