Which of the following are components of DNA replication, PCR, and Chain termination sequencing? I. Ribonucleotides...
Which of the following are components of DNA replication, PCR, and Chain termination sequencing?
I. Ribonucleotides
II. Deoxyribonucleotides
III. Dideoxyribonucleotides
IV. DNA Polymerase
a. II, IV
b. I, III
c. I, II
d. II, III, IV
e. I, II, IV
Homework Answers
Option a is correct. Deoxyribonucleotides and DNA polymerase are the components of DNA replication, PCR, and Chain termination sequencing. Dideoxynucleotides are only used in the chain termination sequencing while ribonucleotide primers are used in DNA replication only.
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Which of the following are components of DNA replication, PCR,
and Chain termination sequencing?
I. Ribonucleotides...
1- Which are the differences between the PCR we used in the lab and DNA replication...
1- Which are the differences between the PCR we used in the lab and DNA replication in human cells? A. PCR replicates specific DNA segments while in human cells all the DNA is replicated B. PCR uses high temperature to dissociate the DNA strands while DNA replication in human cells uses helicase C. PCR uses a high temperature resistant polymerase while the DNA polymerase from human cells would denature at high temperatures D. A and B are true E. A,...
I Q10A. Differentiate between two types of DNA sequencing methodologies, sequencing by chain termination and next...
I Q10A. Differentiate between two types of DNA sequencing methodologies, sequencing by chain termination and next generation sequencing. (5 points)
7.. In the controlled termination method of DNA sequencing, reading the gel from _____ gives the...
7.. In the controlled termination method of DNA sequencing, reading the gel from _____ gives the sequence in the _____ direction; _____ fragments that were terminated _____ in polymerization move faster down the gel a. bottom to top; 5′ to 3′; shorter; early b. top to bottom; 5′ to 3′; longer; early c. bottom to top; 5′ to 3′; longer; later d. top to bottom; 5′ to 3′; shorter; early e. bottom to top; 3′ to 5′; shorter; early 8....
1) What does PCR stand for and what does it do? a. Polymerase Chain Reaction; PCR...
1) What does PCR stand for and what does it do? a. Polymerase Chain Reaction; PCR deletes DNA b. Polymerase Copying Repeats; PCR amplifies DNA c. Polymerase Copying Releats; PCR deletes DNA d. Polymerase Chain Reaction; PCR amplifies DNA 2) During gel electrophoresis, the DNA fragments are separated by ____ a. charge b. DNA fragments cannot be separated c. color d. size 3) Primers are a. double stranded DNA oligonucleotide (fragment) b. double stranded RNA oligonucleotide (fragment) c. single stranded...
What is the sequence of the template DNA used to make this Sanger (chain termination) sequencing...
What is the sequence of the template DNA used to make this Sanger (chain termination) sequencing gel? A C GT a.5' - TTGGCCAAA - 3 6.5' - AAACCGGTT-3' OC. 5'- ATGGACTCA - 3 d. 5'-ACTCAGGTA - 3' e. 5' - TGAGTCCAT - 3'
Which of the following statements about the polymerase chain reaction (PCR) is false? a The DNA...
Which of the following statements about the polymerase chain reaction (PCR) is false? a The DNA ligase used is from a thermophilic bacteria so that it can resist denaturation at high temperatures. b Newly synthesized DNA must be heat-denatured before the next round of DNA synthesis begins. c The boundaries of the amplified DNA segment are determined by the synthetic oligonucleotides used to prime DNA synthesis. d DNA amplified by PCR can be cloned.
When sequencing DNA by chain termination, the terminator nucleotide A) is missing a 2' OH B)is...
When sequencing DNA by chain termination, the terminator nucleotide A) is missing a 2' OH B)is missing a 5' phosphate C)is missing both 2' and 3' OH
Which of the following is true regarding DNA sequencing? (multiple answers possible) a. Next generation sequencing...
Which of the following is true regarding DNA sequencing? (multiple answers possible) a. Next generation sequencing is limited to simultaneous sequencing of 23 different nucleotide chains at a time b. Next generation sequencing or “sequencing by synthesis”, requires ddNTPs c. DNA fragments generated by the Sanger sequencing reaction each contain a primer incorporated at the 5’ end of the nucleotide chain. d. Primers are labeled with different flurophores allowing all 4 chain termination reactions for Sanger sequencing to be combined...
Which of the following make(s) sequencing by the Sanger chain-termination method possible? (Selec...
Which of the following make(s) sequencing by the Sanger chain-termination method possible? (Select all that apply.)12.17 a. Complementary single-stranded nucleic acid sequences can come together to form a duplex molecule. b. Single-stranded nucleic acid molecules can be immobilized on certain types of filter paper. c. A DNA strand whose 3' end terminates in a dideoxynucleotide cannot be elongated. d. Duplex nucleic acid molecules can be separated by size by means of electrophoresis. e. New nucleotides are added only to the...
Which is not one of the elements needed to amplify DNA by polymerase chain reaction (PCR)?...
Which is not one of the elements needed to amplify DNA by polymerase chain reaction (PCR)? Question 16 options: A) Nucleoside triphosphates that serve as the source of the nucleotides A, T, C, and G needed in the synthesis of the new strands of DNA B) A restriction endonuclease enzyme that cleaves DNA at specific locations C) The segment of DNA that must be copied D) A DNA polymerase enzyme that will catalyze the synthesis of a complementary strand of...
I Q10A. Differentiate between two types of DNA sequencing methodologies, sequencing by chain termination and next generation sequencing. (5 points)
What is the sequence of the template DNA used to make this Sanger (chain termination) sequencing gel? A C GT a.5' - TTGGCCAAA - 3 6.5' - AAACCGGTT-3' OC. 5'- ATGGACTCA - 3 d. 5'-ACTCAGGTA - 3' e. 5' - TGAGTCCAT - 3'
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