Ans) People who develop only acute symptoms that are mild usually do not require a physician because the illness resolves spontaneously. However, for some children, a pediatrician may be notified and/or may want to see the child.
- Intestinal infection can lead to dehydration and serious complications, such as kidney failure and sometimes death, if it's not treated.
- You should see your doctor( Physician ) if: You have diarrhea that isn't getting better after four days, or two days for an infant or child. You have a fever with diarrhea.
if you wanted to isolate E. coli from a polymicrobial culture, what would be the best selective media to use? Explain below:
You go to see the doctor. The doctor selects you at random to have a blood test for swine flu, which for the purposes of this exercise we will say is currently suspected to affect 1 in 10,000. The test is 96% accurate, in the sense that the probability of a false positive is 4%. The probability of a false negative is zero. You test positive. What is the new probability that you have swine flu? (round to the nearest...
If Dam methylase is overproduced in E. coli would you expect it to have any effect on the mutation rate of cells? Briefly explain why.
If you have a culture of E. coli at a concentration of 5x10bacteria/mL and you infect them with phage at an MOI of 3, what concentration of phage should you use?
Why do you heat shock your E coli/plasmid mixture and then incubate on ice.
Assume that you are a doctor and have a question about your patient’s treatment. What is the best channel to use to obtain an opinion from a colleague?
You set up a transformation hoping that a strain of E. coli will take up a plasmid containing an ampicillin resistance gene, making the strain able to grow on LB + amp. To see if the transformation worked, you set up four plates: (1) Untransformed E. coli cells on LB plate (2) Untransformed E. coli cells on LB + amp plate (3) Transformed E. coli cells on LB plate (4) Transformed E. coli cells on LB + amp plate. Your...
does E. coli spread out (swarm) from the circular inoculation on a Petri dish. Why or Why not? when you inoculate a petri dish with e coli by only forming a circular inoculation, how is the bacteria moving? it is forming biofilm
How much would you have to dilute 1x108 E. coli cells/ml so that when you plate 200µl you obtain 200 E. coli cells on a LB Agar plate?
E. Coli have been genetically modified to create tRNAs that recognize stop codons. Name an example of a tRNA that is normally not encoded into E. Coli. What stop codon does it target, and what modified amino acid does it have on its tRNA? Why is encoding a mutant amino acid beneficial for studying proteins? Why would encoding a mutant amino acid also be beneficial to make modified proteins?