What is the biosynthetic pathway?
Homework Answers
Biosynthetic pathway: To produce product(s) through different intermediates from a precursor by using different enzyes in each intermediate step.
Mutation blocks the biosynthetic pathway as it is unable to produce the enzyme required in the paathway. Compounds used in the earliest(precursor) of the pathway allow the growth of fewest mutants, compound found in last support almost every mutants growth.
The biosynthetic pathway -
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What is the biosynthetic pathway?
Media Supplement B C D EG Glu A Asn Glu+Asn +...
You are studying the live biosynthetic pathway in bread mold that produces a protein necessary for...
You are studying the live biosynthetic pathway in bread mold that produces a protein necessary for life. Your research group has identified three genes believed to function in the live pathway and has created mutant lines for each: die-21, die-18, and die-1. You have been growing the mutant strains and a wild-type strain on deficient media that does not contain all four of the compounds involved in the live pathway: A, B, C, and D. To determine the order of...
Met-Ala-Arg-Tyr-Ala-Asn-Asn-Glu__Lys-Glu-Leu-Leu-Tyr__Arg-Tyr-Ala-Asn__Phe-Leu-Ala-Asn-Asn-Ile-Gly-Ala-Asn__Ile-Ser__Ile-Asn-Thr-Glu-Arg-Glu-Ser-Thr-Glu-Asp__Ile-Asn__ His-Glu-Arg__Phe-Ala-Thr-His-Glu-Arg-Ser__Thr-Arg-Ile-Gly-Leu-Tyr-Cys-Glu-Arg-Ile-Asp-
Met-Ala-Arg-Tyr-Ala-Asn-Asn-Glu__Lys-Glu-Leu-Leu-Tyr__Arg-Tyr-Ala-Asn__Phe-Leu-Ala-Asn-Asn-Ile-Gly-Ala-Asn__Ile-Ser__Ile-Asn-Thr-Glu-Arg-Glu-Ser-Thr-Glu-Asp__Ile-Asn__ His-Glu-Arg__Phe-Ala-Thr-His-Glu-Arg-Ser__Thr-Arg-Ile-Gly-Leu-Tyr-Cys-Glu-Arg-Ile-Asp-Glu__Leu-Glu-Val-Glu-Leu-Ser__Ser-Ile-Asn-Cys-Glu__His-Glu-__His-Ala-Pro-Pro-Ile-Leu-Tyr__Glu-Ala-Thr-Ser__Val-Ala-Asn-Ile-Leu-Leu-Ala__Cys-Ala-Lys-Glu-__Ala-Asn-Asp__Pro-Glu-Cys-Ala-Asn__Pro-Ile-Glu__Trp-Ile-Thr-His__Phe-Arg-Ile-Glu-Asp__Cys-His-Glu-Glu-Ser-Glu-Cys-Ala-Lys-Glu__Ile-Cys-Glu__Cys-Arg-Glu-Ala-Met__Glu-Val-Glu-Arg-Tyr-Asp-Ala-Tyr__Ala-Asn-Asp__Ser-His-Glu__Ile-Ser__Asp-Glu-Val-Ala-Ser-Thr-Ala-Thr-Glu-Asp__Thr-His-Ala-Thr__Asp-Ile-Ser-Glu-Ala-Ser-Glu-Ser__Leu-Ile-Lys-Glu__His-Glu-Ala-Arg-Thr__Asp-Ile-Ser-Glu-Ala-Ser-Glu__Ser-Leu-Glu-Glu-Pro__Ala-Pro-Asn-Glu-Ala__Ser-Glu-Val-Glu-Arg-Glu__Trp-Glu-Ile-Gly-His-Thr__Gly-Ala-Ile-Asn__Trp-Ile-Leu-Leu__Ala-Arg-Ile-Ser-Glu__Ile-Phe__His-Glu__Lys-Glu-Glu-Pro-Ser__Thr-His-Ile-Ser__Glu-Ala-Thr-Ile-Asn-Gly__Pro-Ala-Thr-Thr-Glu-Arg-Asn__Tyr-Glu-Thr__Ile-Phe__His-Glu__Trp-Trp-Trp-Trp-Ile-Ile-Ile-Ile-Ile-Leu-Leu-Leu-Leu-Leu-Ser-Ser-Ser-Ser__Cys-His-Ala-Asn-Gly-Glu__Ile-Asn__His-Ile-Ser__Leu-Ile-Phe-Glu-Ser-Thr-Tyr-Leu-Glu__His-Glu__Cys-Ala-Asn__Ser-Thr-Ile-Leu-Leu__Arg-Glu-Met-Ala-Ile-Asn__His-Glu-Ala-Leu-Thr-His-Tyr__Ser-Ala-Ser-Ser-Tyr__Ala-Asn-Asp__Ala-Leu-Arg-IleGly-His-Thr 1.) Write out the 1 letter amino acid abbreviation for each of the three-letter amino acid abbreviated words listed in the given sequence. The __ indicates a space in between the words. Use www.expasy.org and other bioinformatic tools to generate the following bioinformatic data for the given polypeptide sequence. You must give the name and link to the program you used to generate the data: 2.) Compute the pI and Mw (isoelectric point and molecular mass, respectively) of...
IIIA. (8 pts) 1. A biosynthetic pathway is shown with 3-phosphoglycerate as the starting point. Fill...
IIIA. (8 pts) 1. A biosynthetic pathway is shown with 3-phosphoglycerate as the starting point. Fill out all of the missing factors. 0 2- ? 0 0 H+ O 0 0 R 0 B NAD+ NADH OH 2 00C NH3+ + H20 OH H coo- HCoo +H3N coo- +H3N Coo- 3-Phospho- glycerate
1. As seen below, A-D refer to intermediates in the arginine biosynthesis pathway. Enzyme 1 Enzyme...
1. As seen below, A-D refer to intermediates in the arginine biosynthesis pathway. Enzyme 1 Enzyme 2 Enzyme 3 Enzyme 4 AB+CƏDŹ arginine A particular mutant strain of Neurospora can grow in minimal media supplemented with intermediate D. It is unable to grow in minimal media alone or in minimal media supplemented with intermediates A, B, or C. This strain has a mutation in the gene encoding: a. Enzyme 1 b. Enzyme 2 c. Enzyme 3 d. Enzyme 4 2....
the one I have selected for the first part was wrong. need help with both parts....
the one I have selected for the first part was wrong. need
help with both parts. Thank you!
Suppose a researcher investigates the pathway of synthesis of compound D. This compound is necessary for growth and survival in many fungi, including Neurospora species. He produces three mutants and places each mutant in each of four different growth supplements. The researcher records the production of compound D in this data table. Minimal medium Supplement A Supplement B Supplement Mutant 1 Mutant...
10. The peptide shown has the amino acid sequence: A. Val-Ser-Ile-Glu-Lys B. Lys-Glu-Ile-Ser-Val C. Thr-Asp-Leu-Gln-Arg D....
10. The peptide shown has the amino acid sequence: A. Val-Ser-Ile-Glu-Lys B. Lys-Glu-Ile-Ser-Val C. Thr-Asp-Leu-Gln-Arg D. Val-Asp-Ile-Glu-Arg 11. Which of the following describes the entire three- dimensional structure of a single polypeptide? A. Secondary structure B. Quaternary structure C. Tertiary structure D. Primary structure 12. What is the primary driving force in the formation of protein tertiary structure? A. Energy released when additional ion pairs are formed. B. The exclusion of non-polar substances from aqueous solution. C. The formation of...
What is the value of D Go' for the pathway A <=> B <=> C <=>...
What is the value of D Go' for the pathway A <=> B <=> C <=> D? Reaction D Go' B <==> A +5 kcal/mol C <==> A +5 kcal/mol C <==> D -15 kcal/mol A. +10 B. +5 C. -10 D. -15 E. -20 I think the answer is E. Can you please explain?
Response To Questions From Part A To B (Note: Use your thought only!! Thanks) Six mutants...
Response To Questions From Part A To B (Note: Use your thought only!! Thanks) Six mutants are isolated, all of which require compound G for growth. The compounds A-E in the biosynthetic pathway to G are known, but their order in the pathway is not known. Each compound is tested for its ability to support the growth ofeach ofthem utants #1-6) In the followingtable apis sign indicatesgrowth anda zero indicates no growth MUTANT COMPOUND TESTED a. Draw the order of...
Im confused with the answer for B. Where did the AUG come from? 13.31 Mutations in...
Im confused with the answer for B. Where did the AUG come
from?
13.31 Mutations in the genes encoding the a and B subunits of hemoglobin lead to blood diseases such as thalassemias and sickle-cell anemia. You have found a family in China in which some members suffer from a new genetic form of anemia. The DNA sequences at the 5' end of the nontemplate strand of the normal and mutant DNA encoding the o subunit of hemoglobin are as...
The genetic code is considered degenerate because most amino acids are encoded by at least two...
The genetic code is considered degenerate because most amino acids are encoded by at least two different codons. Some researchers have hypothesized that during evolution the genetic code has been optimized for its intended use in different organisms. Reseachers can study this by examining the relationship between the frequency with which an amino acid appears in all the proteins in an organism and the total number of codons for an amino acid. An optimized organism would use amino acids in...
IIIA. (8 pts) 1. A biosynthetic pathway is shown with 3-phosphoglycerate as the starting point. Fill out all of the missing factors. 0 2- ? 0 0 H+ O 0 0 R 0 B NAD+ NADH OH 2 00C NH3+ + H20 OH H coo- HCoo +H3N coo- +H3N Coo- 3-Phospho- glycerate
1. As seen below, A-D refer to intermediates in the arginine biosynthesis pathway. Enzyme 1 Enzyme 2 Enzyme 3 Enzyme 4 AB+CƏDŹ arginine A particular mutant strain of Neurospora can grow in minimal media supplemented with intermediate D. It is unable to grow in minimal media alone or in minimal media supplemented with intermediates A, B, or C. This strain has a mutation in the gene encoding: a. Enzyme 1 b. Enzyme 2 c. Enzyme 3 d. Enzyme 4 2....
the one I have selected for the first part was wrong. need
help with both parts. Thank you!
Suppose a researcher investigates the pathway of synthesis of compound D. This compound is necessary for growth and survival in many fungi, including Neurospora species. He produces three mutants and places each mutant in each of four different growth supplements. The researcher records the production of compound D in this data table. Minimal medium Supplement A Supplement B Supplement Mutant 1 Mutant...
10. The peptide shown has the amino acid sequence: A. Val-Ser-Ile-Glu-Lys B. Lys-Glu-Ile-Ser-Val C. Thr-Asp-Leu-Gln-Arg D. Val-Asp-Ile-Glu-Arg 11. Which of the following describes the entire three- dimensional structure of a single polypeptide? A. Secondary structure B. Quaternary structure C. Tertiary structure D. Primary structure 12. What is the primary driving force in the formation of protein tertiary structure? A. Energy released when additional ion pairs are formed. B. The exclusion of non-polar substances from aqueous solution. C. The formation of...
Response To Questions From Part A To B (Note: Use your thought only!! Thanks) Six mutants are isolated, all of which require compound G for growth. The compounds A-E in the biosynthetic pathway to G are known, but their order in the pathway is not known. Each compound is tested for its ability to support the growth ofeach ofthem utants #1-6) In the followingtable apis sign indicatesgrowth anda zero indicates no growth MUTANT COMPOUND TESTED a. Draw the order of...
Im confused with the answer for B. Where did the AUG come
from?
13.31 Mutations in the genes encoding the a and B subunits of hemoglobin lead to blood diseases such as thalassemias and sickle-cell anemia. You have found a family in China in which some members suffer from a new genetic form of anemia. The DNA sequences at the 5' end of the nontemplate strand of the normal and mutant DNA encoding the o subunit of hemoglobin are as...
The genetic code is considered degenerate because most amino acids are encoded by at least two different codons. Some researchers have hypothesized that during evolution the genetic code has been optimized for its intended use in different organisms. Reseachers can study this by examining the relationship between the frequency with which an amino acid appears in all the proteins in an organism and the total number of codons for an amino acid. An optimized organism would use amino acids in...
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