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QUESTION 8 List the components used in PCR versus the components used in qPCR.

QUESTION 1 Which of the following is not valid about how Primers work for PCR Having a sequence that anneals in the range of

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Answer #1

8.

Components used in PCR
a. DNA template(to be copied)
b. Set of primers(forward and reverse primers)
c. dNTP
d. MgCl2
e. Taq polymerase
f. Taq polymerase buffer
g. Water


The components used in qPCR

a. template DNA(to be copied)
b. Set of primers(forward and reverse primers)
c. water
d. dNTP
e. MgCl2
f. Taq polymerase
g. Taq polymerase buffer
h. Fluorescent oligonucleotide probe

1. The correct option is- B

Primers designed should always be complementary to the respective template DNA.
Primers designed should be at least 18-22 bp long and the GC content should be in and around 40-60%.

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