113. Thioglycollate broth that is inoculated and incubated and has a 2 cm cloudy area from...
113. Thioglycollate broth that is inoculated and incubated and has a 2 cm cloudy area from the top with a completely clear area above and below is classified as to oxygen utilization as a/an: (a) aerobic (b) anaerobic (c) facultative (d) no growth (e) microaerophilic
114. Thioglycollate broth that is inoculated and incubated and has a cloudy area just below the top with tiny “string” of cloudiness all the way to the bottom of the tube is classified as to oxygen utilization as a/an: (a) aerobic (b) anaerobic (c) facultative (d) no growth (e) microaerophilic
115. Thioglycollate broth that is inoculated and incubated and has cloudy area of the same “thickness” throughout the tube is classified as to oxygen utilization as a/an: (a) aerobic (b) anaerobic (c) facultative (d) aerotolerant (e) none of these
116. Thioglycollate broth that is inoculated and incubated and has no cloudiness is classified as to oxygen utilization as a/an: (a) aerobic (b) anaerobic (c) facultative (d) no growth (e) micro-aerophilic
Homework Answers
113. As the bacteria has grown 2 cm cloudy area from the top and above and below this cloudy part the broth is clear. This indicates the bacteria as microaerophilic. The Thioglycollate broth contains certain nutrients like Yeast, Casein, beef extracts and Vitamin which aids in the growth of some medically important bacteria. Also it pocess 0.075 percent of Agar to prevent convection currents of atmospheric oxygen and the oxygen is not evenly distributed throughout the broth and its concentration decreases down till bottom of a culture tube. The microaerophilic Bacteria requires little free oxygen at a lower partial pressure than that of atmospheric oxygen, hence in the thioglycollate broth the microaerophilic bacteria will not grow extremely at top of the broth as there the oxygen is abundant and not even very down at the botton of the tube as there is no oxygen present but they grow 2 cm below from the top of the broth where there is less oxygen partial pressure which is optimal aeration required for bacterial growth and survival.
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113. Thioglycollate broth that is inoculated and incubated and
has a 2 cm cloudy area from...
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this is a very tough homework assignment for my environmental biology course so if everyone could...
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biology course so if everyone could please help me I would greatly
appreciate it because I have no access to tutors to check my
work.
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oligono rims (a Why is Fluid Thioglycollate Medium well-adapted for the growth of obligate anaerobes? ds svo ulla dole geab 7605 s lo noisduon 1911 S A) The sodium thiogycollate removes the oxygen that would inhibit the growth of the anaerobe. dossis v aste A B) Anaerobes are fastidious and require the nutrients found in FTM. C) The resazurin promotes growth of anaerobes. donec D) FTM suppresses the growth of aerobes allowing anaerobes to flourish. 6. Although Fluid Thiogycollate Medium...
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Is it possible a bacterium that can only grow at the bottom of a tube of thioglycolate broth is also oxidase-positive? Why, or why not? Select one: O a. Yes, it is absolutely possible. This bacterium is facultative anaerobic, so it uses oxidase to ferment glucose. O b. No, it is not possible. It is an obligate anaerobe, and has no reason to have enzymes needed for aerobic respiration. O c. No, it is not possible. It can only use...
14. Lab Exercise 5-1 is an interesting way to run these test tube experiments without oxygen - seal the tube with a layer of clear mineral oil on top (see the figure in the manual). Based on this figure and the Table of Results for this experiment, if the sealed tube is all green or slightly blue but the unsealed tube has any amount of yellow, what type of energy metabolism does that organism have? a. Oxidative only b. Oxidative...
correct the ones marked wrong, and explain why they are the
right answer
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this is a very tough homework assignment for my environmental
biology course so if everyone could please help me I would greatly
appreciate it because I have no access to tutors to check my
work.
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