A sample of DNA was sequenced using the dideoxy nucleotide method, and the resulting (synthesized) products were resolved on the gel (shown here). What is the correct sequence of the synthesized DNA (=DNA that is shown on the gel) written 5’->3’ direction?
5’-AGTCA -3’
5’-TACGGACTGA -3’
5’-AGTCAGGCAT -3’
5’-ATGCCTGACT -3’
5’-TCAGTCCGTA-3’
Homework Answers
I believe the correct answer to be:
Option C) 5’-AGTCAGGCAT -3’
As the 5' end is at the bottom and 3' incorporation is at the top of the gel.
feel free to leave a comment down below for any further query. good rating would be appreciated if you find my answer helpful. thank you.
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Pleaae answer this with explanation asap.I would really apprecuate
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6. (4 points) Dideoxy sequencing of a fragment of DNA produces the gel pattern shown below, with the and+ to the left of the gel referring to the negative and positive electrodes of the gel, respectively. C T A G Which of the following statements about the gel products and the sequence of the DNA template are correct? The sequence of the template DNA is 5'-GACTCGACTGTACGTGC-3', te letter above...
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Eplgenetic modifications to DNA sequences end resulting alterations in chromatin structure can be analyzed by examining DNA methylation and histone modifications. To examine methylation of a DNA sequence, you treat It with sodium bisulfite. If your original DNA sequence Is: ACAGTCCGTCGGAGCCTGCCAGTCGATCGCACCT and yum sequence after trearment reads ACAGTTCGTCGGAGCTTCTTAGTOSATCGCACTT. Which positions on the original DNA sequence are methylated? (Indicate methylations with an * after the affected nucleotide) b.) When this DNA sequence is replicated, which of these methylations will be transferred...
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I just need the answers to questions 2 and 3. My DNA ladder is
in lane 2 with the yellow arrow pointing to it. Thanks!
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order to see the fragments, a stain is typically added to the gel.
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to a DNA molecular weight marker of known size.
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