Question

6. (4 points) Dideoxy sequencing of a fragment of DNA produces the gel pattern shown below, with the and+ to the left of the gel referring to the negative and positive electrodes of the gel, respectively. C T A G Which of the following statements about the gel products and the sequence of the DNA template are correct? The sequence of the template DNA is 5'-GACTCGACTGTACGTGC-3', te letter above each lane corresponds to the dideoxy nucleotide that was left out of the reaction whose products were run in that lane. The sequence of the template DNA is 5'-GACTCGACTGTACGTGC-3'; the letter above each lane corresponds to the dideoxy nucleotide that was included in the reaction whose products were run in that lane. a· b. c.、The sequence of the template DNA is 5'-GCACGTACAGTCGAGTC-3"; the letter above each lane corresponds to the dideoxy nucleotide that was included irn the reaction whose products were run in that lane. The sequence of the template DNA is 5'-GCACGTACAGTCGAGTC-3"; the letter above each lane corresponds to the dideoxy nucleotide that was left out of the reaction whose products were run in that lane. none of the above are the sequence of the DNA template d· e.
Pleaae answer this with explanation asap.I would really apprecuate it .

Answer 1

6) The sequence of DNA template is 5'-GCACGTACAGTCGAGTC-3'. The letter above each lane corresponds to dideoxy nucleotide that was included in the reaction whose products were run in that lane. (option c).

This is because, the bases in the DNA template strand will be complimentary to those observed in DNA strand.

5) The rate of occurrence of neurofibromatosis is higher than the rate of occurrence of retinoblastoma. This is because, the genes that are mutated to cause neurofibromatosis are more prone to DNA replication errors than those that mutated to cause retinoblastoma, because replication error rates can vary depending on the tissue the gene is found in (option c).