Question

Which of the following are usually located in the "open reading frame" of a gene (CHeck all that apply)

exons

promoter

start codon

5'-UTR

introns

polyadenylation signal

a frameshift mutation

3'-UTR

Answer 1

An open reading frame of a gene is the part of gene from the START codon which is TAC in DNA and to the termination codon. This termination codon is for translation meanwhile UTR are transcribed further downstream than the stop codon.

So that's why Promoter, UTRs on both side aren't part of a ORF. Meanwhile both Introns and Exons are part of a ORF as they are transcribed and lies between a Start and Stop codon. Any frameshift mutation in the gene will also be a part of an ORF as this mutation will change the frame of translation thus it is in an exon.

So the right options are :-

Exons

Start codon

Intron

A frameshift mutation