I want to make 6 samples (each sample 0.5 mL) of BSA ranging from ~100-2000 micrograms/ mL from a stock solution that is 10.0 milligrams/ mL. Show 6 calculated samples and all steps.
I want to make 6 samples (each sample 0.5 mL) of BSA ranging from ~100-2000 micrograms/...
I have a solution of 194 ug/mL. I want to make a 100 uL stock of the solution at 100 ng/uL. How do I do this?
From a 2,000 μg/mL BSA stock, create 1 mL of each of the following stock solutions in 0.02 M phosphate buffer using individual microcentrifuge tubes: 50 μg/mL, 250 μg/mL, 500 μg/mL, 1,000 μg/mL, 1,250 μg/mL, 1,500 μg/mL. Calculate the volume of BSA stock that will be required to make the standard solutions needed to create the BSA standard curve. Be sure to show your work and include the volume of 0.02 M phosphate buffer required to reach a final volume...
Describe how much of each of the following stock components you would use to make 250mL of a solution with all of the components below (final concentration of each component is shown) (Show work) a) 1% (w/v) Stock: Dry powder (FW=58.0) b) 2 micrograms/microliter BSA Stock: 100mg/mL
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Describe in detail how you would make a series of four standards ranging from 1 to 100 ppm given the following constraints. a. Your stock solution is 1000 ppm. b. The available pipets have volumes of 2.00 mL, 3.00 mL, and 5.00 mL. c. The available volumetric flasks have volumes of 10.00 mL, 25.00 mL, 50.00 mL, and 100.00 mL. d. You need to make at least 10.00 mL of each of your four standards.
Sample (gamma globulin) volume (microliter) water (microliter) dye (microliter) absorbance (A595) 100(microgram/ml) 50 550 400 .037 100(microgram/ml) 100 500 400 .076 100(microgram/ml) 150 450 400 .103 100(microgram/ml) 200 400 400 .133 100(microgram/ml) 250 350 400 .156 100(microgram/ml) 300 300 400 .185 100(microgram/ml) 400 200 400 .219 Unknown 25 (microgram/ml) 575 400 .075 Unknown 50 (microgram/ml) 550 400 .12 Unknown 100 (microgram/ml 500 400 .185 1) what is the amount of (in micrograms) of gamma globulin in the diluted unknown samples?...
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so: 1 ml stock 10 ml final -0.5 mL of stock -5 ml final volume While I have used 1:10 as a standard the same rules apply for all dilution factors. Try these problems: x 0.5 X0.5 1. You need to make a 1:25 dilution factor. If you final volume is 25 mL this means that you need_ _mL of stock and mL of solvent to make your final volume of 25 mL. 2. If you needed...
You want to prepare 50 mL of a 20% NaCl salt solution from a 100% stock. Calculate the amount of stock as well as the amount of water you need to use to make this dilution. Assume that you have unlimited volume of the stock.
You have a stock solution that contains 24 mg/ml of protein. You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml. You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below. Std 1 Std 2 Std 3 Std 4 concentration (mg/ml) 12 6 3...
You want to make 500 mL of 10 M NaOH solutions? How much NaOH do you have to weigh out? Walk me through the steps it takes to make this solution? During the weighing out process you weigh out 201.53 NaOH, what is your new molarity? Using your stock solution, you want to make a series of dilutions, you want 25 mL of the following concentrations of NaOH: 0.5 M ,1 M, 3 M, 7 M, 9 M. How much...
1) If you were asked to make 100 mL of 2.4M HCl solution from a stock solution of 6.0M HCl. How many mL of stock would you need? 2) The endpoint of a titration is where the molarity of the Acid equals the Molarity of the base. T/F 3) If after your titration, you discover that the concentration of your base is lower than you thought you made it. How does this affect the Pb(NO3)2 M you determined? the concentration...