Write the sequences of the two 12-residue primers that could be used to amplify the following...
Write the sequences of the two 12-residue primers that could be used to amplify the following DNA segment by PCR.
ATAGGCATGGCCTTAAACGCTAGCTTGCAACGTTACGGTCAATGCCGTAACGTTGCA
Homework Answers
The forward primer and reverse primers are the two primer sequences that can be used to amplify a DNA segment.
The forward primer is complementary to antisense strand of a DNA, which means its sequence is same as the given DNA sequence.
Since we are to find primers of length 12, so the 12 - residues long forward primer has the following sequence :
ATAGGCATGGCC
The above sequence is in 5' - 3' direction, assuming that the DNA sequence provided by you is also in the 5' - 3' direction.
For the case of reverse primer, its sequence is complementary to the sense strand, but in a direction of 5' - 3'.
So this gives the following sequence for the reverse primer :
TGCAACGTTACG
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Write the sequences of the two 12-residue primers that could be
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Pick a pair of primers that you could use to amplify the following double-stranded DNA sequence...
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Pick a pair of primers that you could use to amplify the
following double-stranded DNA sequence using PCR. Note: Both
strands shown below.
5'-CCACTAGGGAGCTACGTAGGCACGGCATTACACGGATAGGCATTAACG-3'
3'-GGTGATCCCTCGATGCATCCGTGCCGTAATGTGCCTATCCGTAATTGC-5'
Pick a pair of primers that you could use to amplify the following double-stranded DNA sequence using PCR. Note: Both strands shown below 5'-CCACTAGGGAGCTACGTAGGCACGGCATTACACGGATAGGCATTAACG-3 3'-GGTGATCCCTCGATGCATCCGTGCCGTAATGTGCCTATCCGTAATTGC-5 5'-CCACTAG-3'; 5'-CGTTAAT-3' 5'-GGTGATC-3'; 5'-GCAATTA-3' 5'-GATCACC-3':5'-CGTTAAT-3' 5'-CCACTAG-3';5'-GCAATTA-3' 5'-GGTGATC-3';5'-CGTTAAT-3'
S-CGT-3 GTG 3. Write in the following sequences to depict them hybridizing/annealing to complementary and antiparallel sequence in the exposed nucleotide chains. 5'-GTG-3 5-CGT-3 5'-ACG-3' | 5 -CAC-3" 5'-AAT[CGTATCAGCAGCAGTG|ACT-3 -3'-TTALGCATAGTCGTCGTCATGA-5'- 3. Two of the four above sequences can be used together as a "primer pair" to PCR amplify the bracketed sequence. In order to determine which two will work, recall that new polynucleotide chains can only be added to on the 3'end. Draw an arrow from the 3' end of...
Which of the following components is NOT used in the technique of PCR to amplify a specific DNA sequence in a mouse genome? O Specific single-stranded DNA primers O Double-stranded genomic mouse DNA O Heat-stable DNA polymerase D DNA ligase
CHAPTER 14: Identify the primer sequences that could be used in this PCR reaction shown below to amplify the target region highlighter in pink (which could be an important gene such as insulin that you want to make many copies of). At this point, the double-stranded DNA has been heated so that the strands are now in a single-strand formation. Be sure to pay attention to the DNA sequence and the 5' 3'ends of the primers. 5' GG CCA A...
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1. Create the primers to amplify the gene of interest. 1. Below is almost the full sequence of the coding strand of the F9 gene (exon 2-exon4) that you found in the online database (NCBI, Genomic sequence F9 gene). Some of the sequence is missing, but you know how many nucleotides are skipped. Design forward and reverse primers 18nt each for PCR that will isolate EXACTLY the sequence that is in bold typeface. Exon 2 = 164 nt Intron 2...
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