2. Identify the following components of the
prokaryotic gene below
(include appropriate numbering on the DNA):
a. Pribnow Box
b. -35 region
c. 5’ untranslated region
d. Template strand
e. Nontemplate strand
f. Start site of transcription
Answer : Components of prokaryotic gene
Pribnow box/TATA box : Pribnow box (TATAAAT) which is present ten base pairs upstream of transcriptional start site (- 10 Sequence )
-35 region ( TTGACG) which is present 35 base pairs upstream of the transcriptional start site
Template strand
Non template strand
Start site of transcription ( which is designated as +1)
2. Identify the following components of the prokaryotic gene below (include appropriate numbering on the DNA):...
Below is a diagram of a transcription unit and the mRNA made from the transcription unit: A H TTGACA DNA TATAAT -35 B -10 С D - Start codon Stop codon mRNA 5' 3' E F G Is this a prokaryotic or eukaryotic transcription unit? prokaryotic What is the name of the cofactor required for RNA polymerase to bind to the promoter? Which letters on the above diagram correspond to the following structures? Promoter Pribnow box Non-template strand Transcriptional start...
Below is the partial coding strand of DNA for a gene containing
an ORF, shown 5' to 3'. Identify the regions, shown 1 to 9,
associated with the following genetic elements. Some elements may
have more than one associated region.
(a) The promoter region
(b) The ribosome binding site
(c) The ribosome binding site consensus
sequence
(d) The start codon
(f) The expected region for the +1
transcription (where the transcript
begins to be made)
(g) Is this a prokaryotic...
Below is a diagram of a transcription unit and the mRNA made from the transcription unit: А H DNA TTGACA TATAAT -35 B -10 C D Start codon Stop codon mRNA 5 E F G Is this a prokaryotic or eukaryotic transcription unit? What is the name of the cofactor required for RNA polymerase to bind to the promoter? Which letters on the above diagram correspond to the following structures? Promoters Pribnow box Non-template strand Transcriptional start site- Open Reading...
I. Use the DNA sequence below, which encodes a prokaryotic gene to answer the following questions. 11 21 31 41 51 71 81 61 91 CGTAATTGAG GAGGTAGTTG ACGTATGAAT AGTTAACGTA CGGGGGGGAA 101 111 121 131 141 ACCCCCCCTT TTTTTTTTTC GAGCAATAAA AGGGTTACAG ATTGCATGCT a) Write down the corresponding sequences, find them in the sequence above and label them: -35 Consensus sequence: _(label as -35) -10 Consensus sequence (Pribnow box): _ __ (label as Pribnow) Shine-Dalgarno sequence in corresponding mRNA: __ (label as SD)...
2. (3 pts) Draw a gene! Draw only the template strand of the DNA. Do not draw the non-template strand. Label the 5' and 3' ends of the DNA. Label the promoter, coding region, and termination site. Draw RNA polymerase halfway through the process of transcription, and the RNA it has produced.
please help, thank you!
The DNA sequence below represents an edited version of the PISTILLATA gene from the model plant Arabidopsis thaliana. The sequence comes from the nontemplate (coding) strand. Use the sequence to answer all questions on this assignment. 9 5'-TTTCTCTCTCTATCTCATCAATGTTACTTTAAAACCAATTTACCTTAATAGGTAAGCTCCTCTTCTTG TTCTTCATATAAATCCACATATCCTCTCCTCCATATCTTAACAATTTCATAGCAAACCCTAAAATTGAGA AAGAGATAGAGAGAGAAAGATGGGTAGAGGAAAGATCGAGATAAAGAGGATAGAGAACGCAAACAA CAGAGTGGTGACGGTATCCTTGCTCTCTTTTTAATCTAAAGTTTCTTTGTCTCAAAAATATTCCATGGC CAAAATCATGTAATAAGTTTTCAATTTCTTTACCAAAAACATGTCAAAAGACCCTTGAATCTTTAACCCT AGATGCAGATAACAACAGGAGATGGGATGATGAGAGATCATGACTCGATTGATCATCGAGATTTTAT AATCTCATCCTGATCAACTCCTATCTATAATATCGTGGTCTTTAGTTTGTCTTTATCAATCTGTGTGTCT TAATCTCAATAAAATATACTCATTAAAATCAAAATCCATAAATACGGACATAAACACGACAGACTTACG -3 0 . What kind of gene features would you expect Arabidopsis thaliana to have, bacterial or eukaryotic? 2. The gene contains a sequence that is recognized by the AP1 transcription...
region is a DNA sequence that regulates transcription. Within a gene, a region is a DNA sequence that encodes RNA and a o coding, barcoding o control, coding o noncoding, control o coding, control During transcription, the DNA template is read in the ___direction. o 3 to 5 oC to N terminal ON to C terminal 0 5' to 3' For genes encoding protein, which of the following is the eukaryotic consensus sequence of the promoter? ATAT o GCGC CGCG...
3. Prokaryotic and eukaryotic gene expression compared. Below is an incomplete table of prokaryotic and eukaryotic gene expression in comparison. Fill in the blank using PPT slides, notes and the textbook. Prokaryotic gene expression Eukaryotic gene expression Overview Steps Transcription and translation Yes Transcription and translation coupled? Gene structure No introns Epigenetic modification (chromosome remodeling) transcription, translation, RNA processing, protein processing Transcription in the nucleus and translation in the cytoplasm Interrupted gene with exons and introns RNAPI, II, III Which...
Consensus sequences that are involved in prokaryotic/bacterial transcription include... A. the -10 consensus sequence or Pribnow box. B. the -35 consensus sequence. C. enahancer sequences. D. Two of the above. E. All of the above.
Please solve each item in a detailed and descriptive way.
Q5. Total 35 pts. Below given single stranded DNA sequence was retrieved from a prokaryote; Promoter region is shown with yellow color Transcription start site is shown with green color Ribosome binding site is shown with blue color 5'ATAGTCGTCGATCGATGGCTTAGCTAGCTTCGATTTCGTAGCTCTGATTAAACGCGCGCATATATCGAT ATCTAGCTAGCTATATTCGCTGATCGCTAGTGTGCGTGATGCTGCTAGGATCAGGTATCGGTCTGATCTA GTATTAGTGCCCGTAGCTGATGCTTCGTCGTAGATCGCTGATTCGCTAATAGGCTGCTAGTCGATGCTGT A3' A) Write the sequnce of double stranded DNA from given single stranded DNA sequence. (5 pts) B) Show template DNA strand used in transcription. (5 pts) C) Write...