What is the net charge on the following peptide in the cell, assuming a buffered intracellular...
What is the net charge on the following peptide in the cell,
assuming a buffered intracellular pH of 7.3?
M-T-N-R-D-G-Q-A-E-G-A-F-Y-W-K-C-S-I-R-L-P-L
Homework Answers
Net charge is zero at ph between 4.2 and 9.7. peptide bonds allign in such a manner that charges due to carboxyl and amino acid n terminus cancel out each other so the net charge remains zero. if it was a charged peptide sequence it would have been unstable and not existed.
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What is the net charge on the following peptide in the cell,
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a. Which spot corresponds to the protein with the largest
molecular weight?
b. When pH = 7, what order would the following peptides elute
from a column filled with cation exchange resin? Size exclusion
column?
Protein 1 – 400 kDa;
10%A 5%E, 5%F, 10% G, 5% H, 10%I, 10%K, 10%L 10%R, 5%P, 5%S, 10%W,
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