Question

The following steps are all involved in genetic recombination: 1. Screening for cells that contain the recombined gene. 2. Cutting the vector with restriction enzyme. 3. Mixing the gene of interest with the vector. 4. Isolating the gene of interest from its original source. 5. Ligating the gene of interest and the vector together. The following sequence of these five steps would be typical: O 1) 1, 2, 3, 4, 5 O2) 2, 3, 5, 1,4 O 3) 5, 4, 3, 2, 1 4) 4, 2, 3, 5, 1 5) 2, 3, 5, 4, 1

Answer 1

Genetic recombination is the process of inserting new genes of interest in the DNA of another cell to synthesize required proteins such as enzymes, hormones, peptides, antibodies, etc.

First, isolate the gene of interest from its original source and cut the vector with the help of specific restriction enzymes.

Then, Mix the gene of interest with vector and Ligate the gene of interest with the vector using enzyme ligases.

Then, you can screen the cells that contain the recombinant gene.

Hence, the correct option is 4) 4, 2, 3, 5, 1