Question

14. Which of the following factors does effect on DNA fragments separation during electrophoresis a. Size b. Electrical charge c. Nucleotides sequences d. All of the above Match the following with q 15-18 a. A necessary cofactor for DNA polymerization b. To break cell membrane open c. Confirm plant-based sample d. Detection of GMO e. Cell membrane lysis 15. Salt solution and heat 16. Primer of CaMw promoter 17. Primer of Nopalin Synthetase virus terminator 18. Magnesium chloride 19- Digestion of two different DNA sequences samples with the same enzyme mix, will result in a. Same band numbers with different patterns b. Same Band profiles c. Band profiles will not match d. A and b

help!

Answer 1

14 a) Size

DNA is negatively charged due to phosphate and get separated on the basis of their size , larger fragment move slowly and small fragment move faster.

15 a) a necessary cofactor for DNA polymerisation - 18 ) magnesium chloride ( many enzyme require magnnesium for proper functioning and activation )

b) to break cell membrane open - 15) salt solution and heat

c) confirm plant based sample -16) plant based sample

d) detection of GMO - 17 ) primer of nopalin synthetase virus terminator

e) cell membrane lysis - 15) salt solution and heat ( heat- denature protein and salt    alter the osmosticum of the cell )

d) a&b

site at which an enzyme make a cut  will be same , each restriction enzyme cut at specific site sequence )

pattern can be different but bands have similar sticky end.