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You want to study the proximity of two residues in clamp region of a protein. Draw...

You want to study the proximity of two residues in clamp region of a protein. Draw a schematic of your protein and label two locations of your fluorophores. Explain how your fret fluorophores would determine if these two residues were close to one another

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144 14 nm 495 mm PET when, in free State Free State. protein A 414 km protein B q ercitat on velength 475 ansas Wavelength, Pif two proteins are in close proximity then the chromophores attached with one protein which is donor if become excited ,it will emit the signal at emission wavelength and the emission frequency is the excitaction frequency of acceptor chromophore and then after excitation the donor chromophore will emit the signal at its emission wavelength but which is different from donor chromophore emission wavelength.

Now if two proteins are distantly placed then the do or molecules will not be excited and then you will only get the emission at the emission wavelength of donor chromophore.

Thank you

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