Question

Part Il Short Amwer Oestions and Problem. There are total of sixty-four 64 10 How does a protein's amino acid sequence infonce the tertiary structure 5 Points le prints in this section 11. A protein was purified to homogeneity. Determination of the mass by gel-filtration chromatography yields 30 d. Gel-fturation chromatography in the presence of 6 Murea yields a 117.d species and a 22-d species. The same protein was treated with mM B-mercaptoethanol and analyzed using SDS-PAGE. There were hands at 141-1117- and O . Describe the quaternary structure of the molecule (10 Points) 12. A mixture of two peptides have the following amino acid sequence: Peptide 1: GAIVYLTM and Peptide 2 EDAEWDLO. Use a specific type of chromatography to separate the ride fragments and give the order of elution buffer. pH. etc. Explain your answer to receive full credit. 17 points)

Answer 1

10. Tertiary structure of a protein lie in its primary structure i.e sequence of amino acids . The type and position of amino acid determines the type of covalent and non-covalent interactions which further determine the tertiary structure .

11. There are 3 subunits in the given protein of 143kd , 117kd and 80kd of which 143 and 80 kd proteins are linked through disulfide bonds and 117kd subunit is linked through non-covalent interactions .

12. Given peptides can be separated by anion exchange chromatography .

Peptide1 : GAIVYLTM (+)

Peptide2 : EDAEWLDQ ( 2-)

We can elute these peptides by cation exchange chromatography so peptide 1 will elute 1st and peptide 2 will elute last .