A total volume of 1 mL of cell suspension was used for this exercise. Blood was...
A total volume of 1 mL of cell suspension was used for this exercise. Blood was diluted 1/10. The cells were then serially diluted using saline.
-How was 1/10 cell suspension made?
-How was 1/100 cell suspension made?
-What is the difference between a 1/100 and 1/1000 cell suspension?
Homework Answers
-What is the difference between a 1/100 and 1/1000 cell suspension?
In this case, we have a less concentrated solution and a higher concentrated solution. The 1/100 means that 1 part of the blood sample is diluted in 100 parts, while in a 1/1000 cell suspension, 1 part of the original sample is diluted in 1000 parts
Add Answer to:
A total volume of 1 mL of cell suspension was used for this
exercise. Blood was...
1. You have a bacterial cell culture with a concentration of 1x109 cells/ml. You need to...
1. You have a bacterial cell culture with a concentration of 1x109 cells/ml. You need to dilute the cells to a concentration of 1x103. What is the correct ratio for this dilution? 2. You add 0.1 ml of a yeast culture to a test tube that contains 9.9 ml of buffer solution. What is the dilution factor of this mixture? 3. In a 1000 µl total dilution volume, the volume of cell culture used is 800 µl. What is the...
6) Medical : Red Blood Cell Volume Total blood volume (in ml) per body weight (in...
6) Medical : Red Blood Cell Volume Total blood volume (in ml) per body weight (in kg) is important in medical research. For healthy adults, the red blood cell mean is about y = 28 ml/kg. Red Blood cell volume that is too low or too high can indicate a medical problem. Suppose that Roger has had seven blood tests, and the results were 32 25 41 35 30 37 29 The sample mean is x 32.7 ml/kg. Let x...
Cells are kept in suspension in RPMI a media for glowing cells. You have 30 mL...
Cells are kept in suspension in RPMI a media for glowing cells. You have 30 mL of a cell suspension at a concentration of 7.2 times 10^6 cells/mL. You need to dilute a portion of these cells such that 100 mu L of the new suspension will deliver 1 times 10^5 cells when plated in the wells of a 96-well microstate plate. How would you dilute some cells so you could prepare 48 such wells? Your answer should account for...
A test tube with a suspension of cells that was diluted to 1:100 (DF 1/100). You...
A test tube with a suspension of cells that was diluted to 1:100 (DF 1/100). You performed a cell count using a hemocytometer chamber. Assuming that the count was 15,14,18,20,22 cells in 5 of 1/25 boxes. What is the suspension concentration?
You started with a cell count of 5.2 times 10^5 cells in a total volume of...
You started with a cell count of 5.2 times 10^5 cells in a total volume of 3ml. You need lo seed a six-well plate with the following numbers. Calculate the volume of cell suspension you will need to transfer into 1 well. Each letter represents a different scenario. a. 1.2 times 10^4 cells/mL and up to 2 mL/well b. 2.5 times 10^5 cells/well and up to 2 mL/well c. 3 times 10^4 cells/well and up to 2 mL/well
Your stock cell concentration after couting your cells is 2 x 10^6 cell/ml. What volume of...
Your stock cell concentration after couting your cells is 2 x 10^6 cell/ml. What volume of cell suspension contains 200,000 cells?
You harvested macrophages and resuspended them in 1 ml of PBS. You then took a small...
You harvested macrophages and resuspended them in 1 ml of PBS. You then took a small aliquot (1 microliter) and made a 1/1000 dilution for counting. The count revealed that there were 1 x 10e3 cells/ml in the diluted sample. Calculate the concentration of cells in the original cell suspension. Note: 10e3 (e=exponential). This program does not display exponentials correctly) 1 x 10E7 1x10E6 1x10E8 1x10E9
The concentration of particles in a suspension is 48 per mL. A 5 mL volume of...
The concentration of particles in a suspension is 48 per mL. A 5 mL volume of the suspension is withdrawn. a. What is the probability that the average number of particles per mL in the withdrawn sample is between 46 and 50? b. If a 10 mL sample is withdrawn, what is the probability that the average number per mL of particles in the withdrawn sample is between 46 and 50? c. How large a sample must be withdrawn so...
1a Your cell suspension is 6.3 x 10^8 cells /mL. Explain how to prepare a tube...
1a Your cell suspension is 6.3 x 10^8 cells /mL. Explain how to prepare a tube of 1 mL with cells at 2 x 10^5 cells/mL. 1b Now that you have your tube of 1 mL with cells at 2 x 10^5/mL, explain how to add 10^4 cells to each of 10 wells. Do you have enough cells?
You calculated the live cell concentration to be 1.5 x 10^6 cells/mL. what is the volume...
You calculated the live cell concentration to be 1.5 x 10^6 cells/mL. what is the volume of cell suspension needed to seed a T-75 flask with 7.5 x 10^5 cells in 10 mLs of media.
6) Medical : Red Blood Cell Volume Total blood volume (in ml) per body weight (in kg) is important in medical research. For healthy adults, the red blood cell mean is about y = 28 ml/kg. Red Blood cell volume that is too low or too high can indicate a medical problem. Suppose that Roger has had seven blood tests, and the results were 32 25 41 35 30 37 29 The sample mean is x 32.7 ml/kg. Let x...
Cells are kept in suspension in RPMI a media for glowing cells. You have 30 mL of a cell suspension at a concentration of 7.2 times 10^6 cells/mL. You need to dilute a portion of these cells such that 100 mu L of the new suspension will deliver 1 times 10^5 cells when plated in the wells of a 96-well microstate plate. How would you dilute some cells so you could prepare 48 such wells? Your answer should account for...
You started with a cell count of 5.2 times 10^5 cells in a total volume of 3ml. You need lo seed a six-well plate with the following numbers. Calculate the volume of cell suspension you will need to transfer into 1 well. Each letter represents a different scenario. a. 1.2 times 10^4 cells/mL and up to 2 mL/well b. 2.5 times 10^5 cells/well and up to 2 mL/well c. 3 times 10^4 cells/well and up to 2 mL/well
Most questions answered within 3 hours.
-
Where is the error in this code sequence?
String s1 = "Hello";
String s2 = "ello";...
asked 11 months ago -
Financial data for Joel de Paris, Inc., for last year
follow:
Joel de Paris, Inc.
Balance...
asked 11 months ago -
Consider this reaction:
Al2(SO4)3 (aq)+ BaCl3
(aq) Al2Cl6 (aq)- +
3BaSO4(s) . What is the...
asked 11 months ago -
Suppose that Savneet is considering increasing her
recent random sample from 20 car rentals to 40...
asked 11 months ago -
Trucks arrive at an unloading terminal at an average rate of 120
per hour.
Trucks arrive...
asked 11 months ago -
Why are methanol and ethanol completely soluble in water while
octanol is not very little soluble....
asked 11 months ago -
A facilities manager at a university reads in a research report
that the mean amount of...
asked 11 months ago -
When the CuSO4 is rehydrated by adding water to the anhydrous
compound, is this an endothermic...
asked 11 months ago -
A ray of sunlight is passing from diamond into crown glass; the
angle of incidence is...
asked 11 months ago -
A block of mass 0.249 kg is placed on top of a light, vertical
spring of...
asked 11 months ago -
how do the kidneys compensate in the presences of acidosis
a) trigger hyperventilate
b) reserve acid...
asked 11 months ago -
Question 501 pts
The rental rate of capital to the firm increases. Which of the
following...
asked 11 months ago