Question

3.13 pts) The restriction enzyme known as Notl recognizes the following sequence: 5-GCGGCCGC-3 3-CGCCGGCG-5 However, if the cytosines in this sequence have been methylated. NotI will not cleave the DNA at this site. For this reason, Net is commonly used to investigate the methylation state of CpG islands. A researcher has studied a gene, which we will call T. that is found in com, and encodes a transporter involved in the uptake of phosphate from the soil. A CpG island is located near the core promoter of T and has a single NetI site. CpG island Core promoter 1500 bp Notl 3800 bp Coding sequence for gene T EcoRI Sall A researcher has isolated DNA samples from four different tissues in a corn plant. The DNA was then digested with the restriction enzymes Sall. Notſ, and EcoRI. and the resulting fragments were separated by gel electrophoresis and “probed" with a complementary sequence to the coding sequence of gene T. Explain the results in the following gel image based on this information. Does this data make sense for the function of the T protein? Explain. 1 2 3 4 5300 bp - DNA from: Lane 1. Leaf tissue Lane 2. Tassels Lane 3. Stem tissue Lane 4. Root tissue 3800 bp

Answer 1

Ans: Cytosines of CpG island not get methylated in root tisses rather than leaf tisses,tassels,stem tissue.

cob Promotor T-gene Sal Noti ECORE 1500 bp 3200 If the issosine (c) of the coG iseland get methylated then Not I enzyme will not leave that site, so then Salls EcoRI cheer cleare during restriction digestion and produce a band of (1500 + 3800) = 5300 bp. And if the (c) of the Cpb iseland not get methylated, the Not I, Sali S ECORI cleare during custoiction digestion and produce band of 1500 op & 3800 bp. from the gel smage, tome dan conclude that, in Root tissues, the updorine (c) of cpb iseland get not get methylated reather them nof tissue, tassels, stem tissue. DNA methylation is essential for silencing, for tirnce specific gene regulatic regulation. & According to the data cue can assume that I - protein, Eurotre in growth of dooks, and development of roots, may be geotropic more ment of root.