Question

Using the GC analysis, can someone help me label the peaks, and figure out if the fat in the unknown is from an animal or vegetable source (#4 under V. Data Analysis)? Please and thank you!

V. Data Analysis 1.) Identify the peaks in each of the two reference samples based on their known composition given in the table and relative elution times (Rt) in the sample chromatogram (see Appendix). Indicate which fatty acids were not found, if any, and explain why their detection might have been difficult. 2.) Compare the peaks in your unknown sample to the two reference samples and identify each of the major fatty acids. Analyze those peaks that can be assigned by Rt to one of the fatty acids present in either of the standard mixtures. The Ri's may be slightly different in each chromatogram. 3.) Calculate the relative amount of each fatty acid by dividing its peak area by the total area of all fatty acids identified in each standard GC (do not include the area of any solvent peaks in the total area). Do the same for each fatty acid identified in the GC of your unknown. 4.) Prepare a table of the fatty acids found in the unknown sample and their relative composition. Compare the composition to that of the two standard samples of vegetable and animal fats. Is the fat in your unknown more likely from an animal or vegetable source? Explain your reasoning. 5.) Include the table in the report and answer all questions below.

Answer 1

A3 f peaks D А в C TG I H % weight in animal fat Aw 2 2% الا 30% Bocov a fatty acid with respect % wt in 3 peaks RT c length to RTS area % area veg fat 3.015 unk 1.87 1.327088 myrisitic 3.816 14:00 acid 5.55 3.938684 palmitic 4.664 16:00 acid 18.38 13.04379 6% palmitolei 5.104 16:01 c acid 1.74 1.234831 5.489 unk 2.7 1.916117 5.594 unk 51.08 36.25009 6.64 18:01 oleic acid 42.91 30.45206 35% linoleic 8 6.806 18:02 acid 16.68 11.83734 50% sum 140.91 100 K+ Sheeti Sheet2 Sheet3 Select destination and press ENTER or choose Paste 41% 11 7% 12 13

Table show RTs of the sample and based on RTs Fatty acids found are assigned. The percentage composition of the fatty acids calculated based on area contribution of each peak. Looking at the composition the sample looks to be neither of vegitable source not of animal. Vegitable fat has composition of oleic acid and linoleic acid 35 and 50% (Sample has their composition as 30 and 11%) if it would be animal source its major components would be 30% palmitic acid 41%  oleic acid while it is 13 % and 30 % respectively