Draw a workflow of the original Sanger Sequencing Method

The sequencing technologies that succeeded the Sanger sequencing method helped to reduce cost dramatically and to improve throughput. What were the critical improvements made by the second generation of sequencers? What were some of the shortcomings introduced and are being addressed by the third generation of sequencers?
You can improve the read length of Sanger Sequencing by changing the enzymatic activity of the original DNA Polymerase. a. Explain the problems with the DNA polymerase and the modifications required. [3 M] b. Name a commercial Sanger Sequencing DNA polymerase with properties in a) [1 M]
In automated DNA sequencing reaction by the Sanger method, which of the following is NOT a component of the reaction? A) fluorescently-labeled deoxynucleotides B) DNA polymerase C) a DNA primer D) the DNA to be sequenced E) none of the above (i.e. all the above are required in the reaction)
The data below were obtained as the result of the Sanger method of DNA sequencing. Determine the DNA sequence. making sure to indicate 5 and 3 ends, and add the sequence for the complementary strand
O The Sanger method of DNA sequencing requires which of the following to be in the reaction tube? Choose one: O A. template DNA O B. deoxyribonucleotides O C. dideoxyribonucleotides O D. A, B, and C are correct. O E. Only A and B are correct. O F. Only A and Care correct. O O O
ddATP ddCTP ddGTP ddTTP The autoradiogram shown was obtained from a DNA sequencing method called Sanger sequencing or dideoxy sequencing. The arrow shows the direction of migration of the DNA samples during electrophoresis. Determine the sequence of the DNA template strand, in the 5' to 3' direction, from this data. 5' – CGTCAATTTAG
Compare Sanger sequencing and NGS 1. Sanger Sequencing: Amount of DNA (high/low)? Sequence mixed DNA (Yes/No)? Efficiency (high/low)? 2. NGS Amount of DNA (high/low)? Sequence mixed DNA (Yes/No)? Efficiency (high/low)?
Sanger sequencing or dideoxy sequencing of DNA causes termination of replication. Why? 18.
Provide four ways that cycle sequencing is an improvement over conventional Sanger sequencing.
The Sanger method of DNA sequencing requires what type of dNTP modification to generate truncated DNA products? How are individual dNTPs identified? How are the different sizes of DNA separated from each other?