Question

Question 12 Part A Which of the following is NOT a characteristic of Okazaki fragments? D They are longer in eukaryotic cells O They are checked for accuracy by DNA polymerase III D They make up the lagging strand of replicated DNA D They are joined together by DNA ligase. D They begin with an RNA primer Submit Provide Feedback

Answer 1

12) They are longer in eukaryotics.

Okazaki fragments are short,newly synthesized DNA fragments that are formed on the lagging strand during DNA replication.Theey are discovered by Reiji and Tsuneko Okazaki.They are complementary to the lagging template strand.Okazaki fragments are between 1000 to 2000 nucleotides long in prokaryots and are roughly 100 to 200 nucleotides long in eukaryotes.

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21) initiation of translation in bacteria occurs when the ribosomal subunit binds the 5' guanine

Eukaryotes are organisms whose cell have a nucleus enclosed within membrane.Bacteria consit of a single cell with simple internal structure.

Translation is the process of conversion of messenger RNA(mRNA) to a sequence of amino acids during protein synthesis.In bacteria translation is begin with initiation factor F-3(IF-3) binds to the 30S ribosomal units.then mRNA binds to 30S ribosomal subunits in such a way that AUG codon lie on the P site and the second codon lie on the A site.Then the tRNA carrying formylated methionine is placed at P site.This specifycity is induced by IF-2 with utilisation of GTP.the IF-1 prevent binding of FMet-tRNA is in A-site.Shinedalgrno sequence in the mRNA guide correct positioning of AUG codon at P-site of 30S ribosome.After binding of FMet-tRNA on P-site ,IF-3,IF-2 and IF-1 are released so that 50S ribosomal unit bind with 30S forming 70S sibosome.The exit site is locsted in 50S.