Question

1. Match the molecule or term with its description

Question Selected Match semi-conservative A. mechanism of replication for viral DNA theta B. mechanism of replication for circular bacterial DNA rolling circle C. mechanism of DNA replication determined by Meselson and Stahl OriC D. where the two new DNA strands are synthesized A-T rich region E. where DNA unravels when initiator proteins bind OriC replication fork F. where replication begins for the bacterial chromosome leading strand G. the new DNA strand made from the template that opens up in the 3’ to 5’ direction okazaki fragment H. pieces of DNA that will make up the lagging strand lagging strand I. the new DNA strand made from the template that opens up in the 5’ to 3’ direction 5’ to 3’ direction J. direction of synthesis of the new DNA strand 3’ to 5’ direction K. direction the template DNA strand is read as the new DNA strand is made hydrogen L. type of bond between the template nucleotide and the next nucleotide to be added to the new DNA strand Also, Can you put this process in order. 1. Initiator proteins bind to a specific DNA element. 2. Primase adds an RNA primer to the initiation site. 3. DNA ligase joins DNA fragments together. 4. A-T rich region of DNA denatures. 5. DNA polymerase III alpha subunit adds the complementary nucleotide to the 3' end of the primer and continues to do so. 6. DNA helicase unwinds DNA and single-strand binding protein attach to DNA (single strand). 7. DNA polymerase I replaces the RNA with DNA with its 5' to 3' polymerase activity. 8. DNA polymerase III epsilon subunit corrects mistakes (mismatches) that were recently added 9. Okazaki fragments are synthesized.

Answer 1

Question	Selected Match
semi-conservative	C. mechanism of DNA replication determined by Meselson and Stahl
theta	B.   mechanism of replication for circular bacterial DNA
rolling circle	A. mechanism of replication for viral DNA
OriC	F. where replication begins for the bacterial chromosome
A-T rich region	E. where DNA unravels when initiator proteins bind OriC
replication fork	D. where the two new DNA strands are synthesized
leading strand	I. the new DNA strand made from the template that opens up in the 5’ to 3’ direction
okazaki fragment	H. pieces of DNA that will make up the lagging strand
lagging strand	G. the new DNA strand made from the template that opens up in the 3’ to 5’ direction
5’ to 3’ direction	J. direction of synthesis of the new DNA strand
3’ to 5’ direction	K. direction the template DNA strand is read as the new DNA strand is made
hydrogen – The correct answer is phosphodiester. Plz do chek and let me know… except this all the above are matched.	L. type of bond between the template nucleotide and the next nucleotide to be added to the new DNA strand

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